Characterization of oral treponemes isolated from human periodontal pockets

Takeyoshi Koseki, I. Ishikawa, M. Umeda, Y. Benno

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A total of 90 clinical strains of oral treponemes was isolated from subgingival plaque in patients with periodontal disease. They were characterized by biochemical means as well as cell enzyme, protein analysis using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and DNA dot blot hybridization. Sixty strains were isolated on Medium 10 (M10), which was fundamentally serum‐free. The remainder were isolated on serum‐containing media. Isolates were divided into 6 groups according to their biochemical characteristics. Three of the 6 groups were asaccharolytic, and 2 of these 3 groups were Treponema denticola and “Treponema vincenti”. The other 3 groups were saccharolytic and further divided into 9 subgroups. The analyses of cell enzyme, cell protein and dot blot hybridization with the DNA probe of Treponema socranskii indicated that all the saccharolytic groups were T. socranskii or closely related species. This study indicated that the newly characterized saccharolytic oral treponemes could be identified using M10 from the human periodontal pocket.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalOral Microbiology and Immunology
Volume10
Issue number5
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Keywords

  • classification
  • medium 10
  • oral spirochete
  • subgingival plaque
  • treponeme

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

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