TY - JOUR
T1 - Characterization of oligosaccharides in milk of bearded seal (Erignathus barbatus)
AU - Urashima, Tadasu
AU - Nakamura, Tadashi
AU - Nakagawa, Daisuke
AU - Noda, Makoto
AU - Arai, Ikichi
AU - Saito, Tadao
AU - Lydersen, Christian
AU - Kovacs, Kit M.
N1 - Funding Information:
The collection of the seal milk was financed by the National Research Council of Norway, in co-operation with the Norwegian Polar Institute. In addition, this study was supported by a grant-in Aid for Scientific Research (B) in Japan, grant number 13575026 and also by a Grant from The 21st Century COE Program (A-1), from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2004/5
Y1 - 2004/5
N2 - Carbohydrates were extracted from milk of a bearded seal, Erignathus barbatus (Family Phocidae). Free neutral oligosaccharides were separated by gel filtration, anion-exchange chromatography and preparative thin layer chromatography, while free acidic oligosaccharides were separated by gel filtration and then purified by ion exchange chromatography, gel filtration and high performance liquid chromatography. Oligosaccharide structures were determined by 1H-NMR spectroscopy. The structures of the neutral oligosaccharides were as follows; lactose, 2′-fucosyllactose, lacto-N-fucopentaose IV, difucosyl lacto-N-neohexaose and difucosyl decasaccharide which contained a lacto-N-neohexaose unit as well as an additional Gal(β1-4)GlcNAc(β1-3) unit and two residues of non-reducing Fuc(α1-2). The acidic oligosaccharides were thought to contain an Neu5Ac(α2-6) residue linked to GlcNAc or a sulfate linked to Gal at OH-3. The sialyl oligosaccharides and sulfated oligosaccharides had a lacto-N-neohexaose unit and two non-reducing Fuc(α1-2) residues and some of them had in addition one or two Gal(β1-4)GlcNAc(β1-3) units. The milk oligosaccharides of the bearded seal were compared to those of the harbour seal, which had been studied previously.
AB - Carbohydrates were extracted from milk of a bearded seal, Erignathus barbatus (Family Phocidae). Free neutral oligosaccharides were separated by gel filtration, anion-exchange chromatography and preparative thin layer chromatography, while free acidic oligosaccharides were separated by gel filtration and then purified by ion exchange chromatography, gel filtration and high performance liquid chromatography. Oligosaccharide structures were determined by 1H-NMR spectroscopy. The structures of the neutral oligosaccharides were as follows; lactose, 2′-fucosyllactose, lacto-N-fucopentaose IV, difucosyl lacto-N-neohexaose and difucosyl decasaccharide which contained a lacto-N-neohexaose unit as well as an additional Gal(β1-4)GlcNAc(β1-3) unit and two residues of non-reducing Fuc(α1-2). The acidic oligosaccharides were thought to contain an Neu5Ac(α2-6) residue linked to GlcNAc or a sulfate linked to Gal at OH-3. The sialyl oligosaccharides and sulfated oligosaccharides had a lacto-N-neohexaose unit and two non-reducing Fuc(α1-2) residues and some of them had in addition one or two Gal(β1-4)GlcNAc(β1-3) units. The milk oligosaccharides of the bearded seal were compared to those of the harbour seal, which had been studied previously.
KW - Bearded seal
KW - Erignathus barbatus
KW - Milk oligosaccharides
KW - Neutral oligosaccharides
KW - Phocidae
KW - Sialyl oligosaccharides
KW - Sulfated oligosaccharides
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U2 - 10.1016/j.cbpc.2003.12.009
DO - 10.1016/j.cbpc.2003.12.009
M3 - Article
C2 - 15142532
AN - SCOPUS:2442431574
VL - 138
SP - 1
EP - 18
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 1
ER -