Characterization of new conjugated metabolites in bile of rats administered 24,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3

Tatsuya Higashi, Kanako Miura, Ryuta Kikuchi, Kazutake Shimada, Hiroko Hiyamizu, Hidenori Ooi, Yoshiharu Iwabuchi, Susumi Hatakeyama, Noboru Kubodera

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The characterization of new conjugated vitamin D metabolites in rat bile was performed using HPLC, liquid chromatography/tandem mass spectrometry combined derivatization, and GC-MS. After the administration of 24,25- dihydroxyvitamin D3 to rats, 23,25-dihydroxy-24-oxovitamin D3 23- glucuronide, 3-epi-24,25-dihydroxyvitamin D3 24-glucuronide, and 24,25- dihydroxyvitamin D3 3-sulfate were obtained as new biliary metabolites together with 24,25-dihydroxyvitamin D3 3- and 24-glucuronides. The above metabolites, except 24,25-dihydroxyvitamin D3 3-glucuronide, were obtained from rats dosed with 25-hydroxyvitamin D3. 23,25-Dihydroxyvitamin D3 23- glucuronide was also obtained from the bile of rats administered 25- hydroxyvitamin D3 in addition to its 3-glucuronide, 25-glucuronide, and 3- sulfate. Thus, it was found that 24,25-dihydroxyvitamin D3 and 25- hydroxyvitamin D3 were directly conjugated as glucuronide and sulfate, whereas at the C-23 position, they were hydroxylated and then conjugated. Furthermore, we found that the C-3 epimerization acts as one of the important pathways in vitamin D metabolism. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)281-294
Number of pages14
JournalSteroids
Volume65
Issue number5
DOIs
Publication statusPublished - 2000 May
Externally publishedYes

Keywords

  • 24,25-Dihydroxyvitamin D
  • 25-Hydroxyvitamin D
  • 3-Epi-24,25- dihydroxyvitamin D 24-glucuronide
  • Conjugated metabolite
  • Liquid chromatography tandem mass spectrometry
  • Rat bile

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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