Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies

Masafumi Nakayama, Norihiro Harada, Ko Okumura, Hideo Yagita

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

In the present study, we characterized murine TWEAK and its receptor (Fn14) by generating cDNA transfectants and specific monoclonal antibodies (mAbs). Recombinant murine TWEAK bound to murine Fn14-transfected L5178Y (mFn14/L5178Y) cells and induced cell death. Some anti-human Fn14 mAbs we previously generated strongly cross-reacted with murine Fn14 and induced cell death in mFn14/L5178Y cells. Murine TWEAK-transfected L5178Y cells expressed murine TWEAK on cell surface and secreted functional TWEAK, which were detected by a newly generated anti-murine TWEAK mAb (MTW-1). Although thioglycolate-elicited murine peritoneal macrophages did not express a detectable level of TWEAK on their surface, they secreted functional TWEAK that was cytotoxic against mFn14/L5178Y cells and neutralized by MTW-1. The anti-murine TWEAK and Fn14 mAbs will be useful for further investigating the physiological and pathological functions of TWEAK and Fn14.

Original languageEnglish
Pages (from-to)819-825
Number of pages7
JournalBiochemical and biophysical research communications
Volume306
Issue number4
DOIs
Publication statusPublished - 2003 Jul 11

Keywords

  • Cell death
  • Fn14
  • Macrophage
  • Murine
  • TWEAK

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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