TY - JOUR
T1 - Characterization of MRSA transmission in an emergency medical center by sequence analysis of the 3′-end region of the coagulase gene
AU - Kanemitsu, K.
AU - Yamamoto, H.
AU - Takemura, H.
AU - Kaku, M.
AU - Shimada, J.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - The distribution of methicillin-resistant Staphylococcus aureus (MRSA) isolates at the St. Marianna University affiliated emergency medical center (EMC) was studied by sequence analysis of the 3′-end region of the coagulase gene. We collected a total of 42 MRSA isolates, consisting of 20 strains from the hospital environment, 13 strains from the nostrils or fingers of medical staff, and 9 strains from inpatients in the EMC. We compared our results with those from 27 stock strains of known coagulase serotype and 2 strains reported in the literature. All 69 strains tested have four to six tandem repeats in the 3′-end region of the coagulase gene. Among the 42 MRSA isolates collected, the base sequence of the 3′-end region of the coagulase gene was identical in 28 of them (67%). The number of isolates originating from the hospital environment, medical staff, and patients, respectively, that were identical to this representative strain were 18 (90%), 6 (46%), and 4 (44%). Phylogenetic analysis using the DNA sequences of the tandem repeat region demonstrated that almost all strains from the patients formed a concordant cluster with the representative strain from the hospital ward. We also assessed the value of sequence analysis of the 3′-end region of the coagulase gene as an epidemiological marker. Our results indicate that sequence analysis of the 3′-end region of the coagulase gene of MRSA may be a potent epidemiologic typing system.
AB - The distribution of methicillin-resistant Staphylococcus aureus (MRSA) isolates at the St. Marianna University affiliated emergency medical center (EMC) was studied by sequence analysis of the 3′-end region of the coagulase gene. We collected a total of 42 MRSA isolates, consisting of 20 strains from the hospital environment, 13 strains from the nostrils or fingers of medical staff, and 9 strains from inpatients in the EMC. We compared our results with those from 27 stock strains of known coagulase serotype and 2 strains reported in the literature. All 69 strains tested have four to six tandem repeats in the 3′-end region of the coagulase gene. Among the 42 MRSA isolates collected, the base sequence of the 3′-end region of the coagulase gene was identical in 28 of them (67%). The number of isolates originating from the hospital environment, medical staff, and patients, respectively, that were identical to this representative strain were 18 (90%), 6 (46%), and 4 (44%). Phylogenetic analysis using the DNA sequences of the tandem repeat region demonstrated that almost all strains from the patients formed a concordant cluster with the representative strain from the hospital ward. We also assessed the value of sequence analysis of the 3′-end region of the coagulase gene as an epidemiological marker. Our results indicate that sequence analysis of the 3′-end region of the coagulase gene of MRSA may be a potent epidemiologic typing system.
KW - Coagulase gene
KW - Epidemiology
KW - Methicillin-resistant Staphylococcus aureus
KW - Sequence analysis
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U2 - 10.1007/s101560170030
DO - 10.1007/s101560170030
M3 - Article
C2 - 11406753
AN - SCOPUS:0035038210
VL - 7
SP - 22
EP - 27
JO - Journal of Infection and Chemotherapy
JF - Journal of Infection and Chemotherapy
SN - 1341-321X
IS - 1
ER -