Abstract
Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.
| Original language | English |
|---|---|
| Pages (from-to) | 561-567 |
| Number of pages | 7 |
| Journal | MICROBIOLOGY and IMMUNOLOGY |
| Volume | 33 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1989 |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Virology
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Cite this
Yaegashi, N., Tada, K., Nagata, K., & Sugamura, K. (1989). Characterization of Monoclonal Antibodies against Human Parvovirus B19. MICROBIOLOGY and IMMUNOLOGY, 33(7), 561-567. https://doi.org/10.1111/j.1348-0421.1989.tb02006.x