Abstract
Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.
Original language | English |
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Pages (from-to) | 561-567 |
Number of pages | 7 |
Journal | MICROBIOLOGY and IMMUNOLOGY |
Volume | 33 |
Issue number | 7 |
DOIs | |
Publication status | Published - 1989 |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Virology