Characterization of Monoclonal Antibodies against Human Parvovirus B19

Nobuo Yaegashi; Kohtaro Tada; Kinnya Nagata; Kazuo Sugamura

doi:10.1111/j.1348-0421.1989.tb02006.x

Characterization of Monoclonal Antibodies against Human Parvovirus B19

Nobuo Yaegashi, Kohtaro Tada, Kinnya Nagata, Kazuo Sugamura

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.

Original language	English
Pages (from-to)	561-567
Number of pages	7
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	33
Issue number	7
DOIs	https://doi.org/10.1111/j.1348-0421.1989.tb02006.x
Publication status	Published - 1989

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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author = "Nobuo Yaegashi and Kohtaro Tada and Kinnya Nagata and Kazuo Sugamura",

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AU - Yaegashi, Nobuo

AU - Tada, Kohtaro

AU - Nagata, Kinnya

AU - Sugamura, Kazuo

PY - 1989

Y1 - 1989

N2 - Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.

AB - Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.

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JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

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Characterization of Monoclonal Antibodies against Human Parvovirus B19

Abstract

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