Characterization of Monoclonal Antibodies against Human Parvovirus B19

Nobuo Yaegashi, Kohtaro Tada, Kinnya Nagata, Kazuo Sugamura

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.

Original languageEnglish
Pages (from-to)561-567
Number of pages7
JournalMICROBIOLOGY and IMMUNOLOGY
Volume33
Issue number7
DOIs
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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