Characterization of leaf blade- and leaf sheath-associated bacterial communities and assessment of their responses to environmental changes in CO2, temperature, and nitrogen levels under field conditions

Seishi Ikeda, Takeshi Tokida, Hirofumi Nakamura, Hidemitsu Sakai, Yasuhiro Usui, Takashi Okubo, Kanako Tago, Kentaro Hayashi, Yasuyo Sekiyama, Hiroshi Ono, Satoru Tomita, Masahito Hayatsu, Toshihiro Hasegawa, Kiwamu Minamisawa

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    Rice shoot-associated bacterial communities at the panicle initiation stage were characterized and their responses to elevated surface water-soil temperature (ET), low nitrogen (LN), and free-air CO2 enrichment (FACE) were assessed by clone library analyses of the 16S rRNA gene. Principal coordinate analyses combining all sequence data for leaf blade- and leaf sheathassociated bacteria revealed that each bacterial community had a distinct structure, as supported by PC1 (61.5%), that was mainly attributed to the high abundance of Planctomycetes in leaf sheaths. Our results also indicated that the community structures of leaf blade-associated bacteria were more sensitive than those of leaf sheath-associated bacteria to the environmental factors examined. Among these environmental factors, LN strongly affected the community structures of leaf blade-associated bacteria by increasing the relative abundance of Bacilli. The most significant effect of FACE was also observed on leaf blade-associated bacteria under the LN condition, which was explained by decreases and increases in Agrobacterium and Pantoea, respectively. The community structures of leaf blade-associated bacteria under the combination of FACE and ET were more similar to those of the control than to those under ET or FACE. Thus, the combined effects of environmental factors need to be considered in order to realistically assess the effects of environmental changes on microbial community structures.

    Original languageEnglish
    Pages (from-to)51-62
    Number of pages12
    JournalMicrobes and environments
    Volume30
    Issue number1
    DOIs
    Publication statusPublished - 2015

    Keywords

    • FACE
    • Nitrogen
    • Planctomycetes
    • Rice phyllosphere
    • Temperature

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Soil Science
    • Plant Science

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