Characterization of iris pigment epithelial cell for auto cell transplantation

Toshiaki Abe, Hiroshi Tomita, Toshifumi Ohashi, Katsura Yamada, Yoshiyuki Takeda, Keiko Akaishi, Madoka Yoshida, Masami Sato, Makoto Tamai

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

To establish auto iris pigment epithelial (IPE) transplantation, we characterized the properties of IPE cells and the method of culture using auto serum. Monkey and human IPE cells were obtained and cultured in several conditions, using auto, mouse, rabbit, bovine, or human serum. Immunocytochemical study was performed to confirm that the cells were epithelial in origin. The proliferation rate of the IPE was also calculated from fresh human IPE cells, which were obtained during filtering glaucoma surgery. Proliferation rate was also compared to that of retinal pigment epithelial (RPE) cells. Reverse-transcriptase and polymerase chain reaction for melanogenesis was performed, and the amount of pigment in the IPE cells was also calculated. Mouse and rabbit sera were not effective for the monkey IPE cell culture. Conversely, the cells grew well in the medium with auto, bovine, or human serum. Human IPE cells grew exponentially by the described methods and reached to 60,000 cells after about 4-5 weeks. When we compared them by proliferation rate, IPE cells were less proliferative than RPE cells. The gene expression for melanogenesis and the amount of pigment in the IPE gradually decreased through successive passages. Transplantation has been tried for the treatment of age-related macular degeneration using RPE from fetus or from eye bank eyes. However, focal rejection may play an important role in the clinical results. The establishment of auto IPE cell transplantation may improve the problem of rejection. In the present study, we established auto IPE cell culture using auto serum. The cultured IPE cell showed pigment epithelial cell properties until around five passages in both human and monkey.

Original languageEnglish
Pages (from-to)501-510
Number of pages10
JournalCell Transplantation
Volume8
Issue number5
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • Auto cell transplantation
  • Iris pigment epithelium
  • Monkey
  • Rejection

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Fingerprint Dive into the research topics of 'Characterization of iris pigment epithelial cell for auto cell transplantation'. Together they form a unique fingerprint.

  • Cite this

    Abe, T., Tomita, H., Ohashi, T., Yamada, K., Takeda, Y., Akaishi, K., Yoshida, M., Sato, M., & Tamai, M. (1999). Characterization of iris pigment epithelial cell for auto cell transplantation. Cell Transplantation, 8(5), 501-510. https://doi.org/10.1177/096368979900800505