TY - JOUR
T1 - Characterization of grown-in dislocations in high-quality glucose isomerase crystals by synchrotron monochromatic-beam X-ray topography
AU - Suzuki, Ryo
AU - Koizumi, Haruhiko
AU - Kojima, Kenichi
AU - Fukuyama, Seijiro
AU - Arai, Yasutomo
AU - Tsukamoto, Katsuo
AU - Suzuki, Yoshihisa
AU - Tachibana, Masaru
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/6/15
Y1 - 2017/6/15
N2 - High quality glucose isomerase (GI) single crystals are grown by using chemical cross-linked seed crystals. The crystal structure is an orthorhombic system in which the molecular arrangement is close to a body-centered cubic (bcc) one. The crystal defects, especially dislocations, in GI crystals are experimentally characterized by synchrotron monochromatic-beam X-ray topography. Two straight dislocations are clearly observed, which originate from the interface between the cross-linked seed crystal and the grown crystal. From the invisibility criterion of the dislocation images, it is experimentally identified that they are close to be of pure edge character with the Burgers vector of [1 1¯ 1] which is typical one in bcc metal crystals. Moreover, bead-like contrasts along the dislocation images and the equal-thickness fringes, related to Pendellösung fringes, at crystal edges are clearly observed, which have never been observed in other protein crystals so far. These contrasts can attributed to the dynamical diffraction effect which has been often observed in high-quality crystals such as Si. Thus it seems that the perfection of GI crystals shown in this paper is extremely high compared with other protein crystals reported so far.
AB - High quality glucose isomerase (GI) single crystals are grown by using chemical cross-linked seed crystals. The crystal structure is an orthorhombic system in which the molecular arrangement is close to a body-centered cubic (bcc) one. The crystal defects, especially dislocations, in GI crystals are experimentally characterized by synchrotron monochromatic-beam X-ray topography. Two straight dislocations are clearly observed, which originate from the interface between the cross-linked seed crystal and the grown crystal. From the invisibility criterion of the dislocation images, it is experimentally identified that they are close to be of pure edge character with the Burgers vector of [1 1¯ 1] which is typical one in bcc metal crystals. Moreover, bead-like contrasts along the dislocation images and the equal-thickness fringes, related to Pendellösung fringes, at crystal edges are clearly observed, which have never been observed in other protein crystals so far. These contrasts can attributed to the dynamical diffraction effect which has been often observed in high-quality crystals such as Si. Thus it seems that the perfection of GI crystals shown in this paper is extremely high compared with other protein crystals reported so far.
KW - A1. Characterization
KW - A1. Defects
KW - A1. X-ray topography
KW - A2. Seed crystals
KW - B1. Proteins
UR - http://www.scopus.com/inward/record.url?scp=85007110614&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007110614&partnerID=8YFLogxK
U2 - 10.1016/j.jcrysgro.2016.10.047
DO - 10.1016/j.jcrysgro.2016.10.047
M3 - Article
AN - SCOPUS:85007110614
VL - 468
SP - 299
EP - 304
JO - Journal of Crystal Growth
JF - Journal of Crystal Growth
SN - 0022-0248
ER -