Characterization of duck microsatellite repeat sequences

Hideaki Takahashi, Masahiro Satoh, Mitsuru Minezawa, Tresnawati Purwadaria, Hardi Prasetyo

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We cloned duck microsatellite repeat sequences from genomic DNA of an Indonesian native duck to evaluate the genetic relationships among native breeds of duck. A (CA)n-enriched library was constructed us ng the method of Takahashi et al. (1996) with modifications. In the library, 8.3% of the clones were CA-positive. The average number of uninterrupted (CA)n repeats among 22 duck microsatellites was 11.4. Fifteen clones which could be developed as PCR primer pairs to detect (CA)n, repeat length polymorphisms were identified. They should provide a valuable tool for studies related to biodiversity and population structure in duck.

Original languageEnglish
Pages (from-to)217-219
Number of pages3
JournalJapan Agricultural Research Quarterly
Volume35
Issue number4
DOIs
Publication statusPublished - 2001 Oct

Keywords

  • DNA
  • Indonesia

ASJC Scopus subject areas

  • Biotechnology
  • Ecology
  • Animal Science and Zoology
  • Agronomy and Crop Science

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