TY - JOUR
T1 - Characterization of Brassica S-haplotypes lacking S-locus glycoprotein
AU - Suzuki, Tohru
AU - Kusaba, Makoto
AU - Matsushita, Masanori
AU - Okazaki, Keiichi
AU - Nishio, Takeshi
N1 - Funding Information:
We wish to thank Drs. D. Astley and D. Ockendon for providing plant materials, Prof. T.-H. Kao for critical reading of our manuscript, and Dr. R. Ohsawa for suggestions for the statistical analysis. This work was supported by a grant from the Science and Technology Agency of Japan (T.N.), Grants-in-Aid for special research on Priority Areas (no. 10158202) from the Ministry of Education, Science, Culture and Sports, Japan (T.S.), and a Grant-in-Aid (Bio Design Program) from the Ministry of Agriculture, Forestry and Fisheries, Japan (M.K.).
PY - 2000/9/29
Y1 - 2000/9/29
N2 - Self-incompatibility (SI) in Brassica is regulated by a single multi-allelic locus, S, which contains highly polymorphic stigma-expressed genes, SLG and SRK. While SRK is shown to be the determinant of female SI specificity, SLG is thought to assist the function of SRK. Here we report that the SLG genes of self-incompatible S18 and S60 homozygotes of Brassica oleracea have an in-frame stop codon and a 23 bp deletion resulting in a frame-shift, respectively. The finding that these SLG genes do not encode functional SLG proteins suggests that SLG is not essential for SI. The possible role of SLG in SI was discussed. Copyright (C) 2000 Federation of European Biochemical Societies.
AB - Self-incompatibility (SI) in Brassica is regulated by a single multi-allelic locus, S, which contains highly polymorphic stigma-expressed genes, SLG and SRK. While SRK is shown to be the determinant of female SI specificity, SLG is thought to assist the function of SRK. Here we report that the SLG genes of self-incompatible S18 and S60 homozygotes of Brassica oleracea have an in-frame stop codon and a 23 bp deletion resulting in a frame-shift, respectively. The finding that these SLG genes do not encode functional SLG proteins suggests that SLG is not essential for SI. The possible role of SLG in SI was discussed. Copyright (C) 2000 Federation of European Biochemical Societies.
KW - Frame-shift
KW - In-frame stop codon
KW - SLG
KW - SRK
KW - Self-incompatibility
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U2 - 10.1016/S0014-5793(00)02048-2
DO - 10.1016/S0014-5793(00)02048-2
M3 - Article
C2 - 11018531
AN - SCOPUS:0034730835
SN - 0014-5793
VL - 482
SP - 102
EP - 108
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -