Characterization of B Cells Expressing Recombination Activating Genes in Germinal Centers of Immunized Mouse Lymph Nodes

Masaki Hikida, Masaharu Mori, Teruyuki Kawabata, Toshiyuki Takai, Hitoshi Ohmori

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

Products of recombination activating genes (RAG-1 and RAG-2) involved in the rearrangement of Ig genes have been shown to be expressed only in immature stages of B cells. However, we have recently found that RAG genes were reexpressed in mature mouse B cells activated in vitro and in germinal centers (GCs) of immunized mouse lymph nodes (LNs). Here, we report that RAG transcripts and their proteins were expressed in parallel with the formation of GCs in popliteal LNs from mice immunized in the hind footpads. Immunocytochemical analysis revealed that RAG+ B cells were localized within GCs and were present as apoptotic tingible body cells. RAG expression is not considered a nonspecific result of apoptosis, since apoptotic B cells generated by surface Ig-engagement did not express RAG genes. These results suggest a novel role of RAG products in the differentiation of B cells in GCs.

Original languageEnglish
Pages (from-to)2509-2512
Number of pages4
JournalJournal of Immunology
Volume158
Issue number6
Publication statusPublished - 1997 Mar 15
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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