Characterization of an immortalized hepatic stellate cell line established from metallothionein-null mice

Nobuhiko Miura, Yoshitaka Kanayama, Wakako Nagai, Tatsuya Hasegawa, Yoshiyuki Seko, Toshiyuki Kaji, Akira Naganuma

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Hepatic stellate (HS) cells were isolated from the livers of metallothionein (MT)-null and control mice and used to establish IMS/MT(-) and IMS/N cell lines, respectively, using SV40 virus transformation. Cellular morphology, incorporation of vitamin A and expression of α-SMA, desmin and SV40 T-antigen were used to confirm that both cell lines were immortal HS cells. The growth rates of both cell lines were similar and there was little difference between cell line sensitivity to zinc. MT-null IMS/MT(-) cells were more sensitive to cadmium and mercury, although both cell lines accumulated almost equal amounts of cadmium during a 24-hr culture period. As HS cells play an important role in hepatic fibrosis and are activated by heavy metals such as cadmium or reactive oxygen, the MT-null HS cell line derived in this study should be a useful experimental model for examination of the role of MT in HS cell activation.

Original languageEnglish
Pages (from-to)391-398
Number of pages8
JournalJournal of Toxicological Sciences
Volume31
Issue number4
DOIs
Publication statusPublished - 2006 Oct

Keywords

  • Cadmium
  • Hepatic stellate cells
  • Immortalization
  • Metallothionein-null cells

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Characterization of an immortalized hepatic stellate cell line established from metallothionein-null mice'. Together they form a unique fingerprint.

Cite this