Characterization of an Extended-Spectrum β-Lactamase from Escherichia coli Mediated by a Plasmid

Matsuhisa Inoue; Aisaku Fuse; Keizo Yamaguchi; Harushige Kanno; Mitsuo Kaku; Takashi Inamatsu; Tsutomu Nagahama; Masato Yoshida; Masato Nonoyama; Ryoichi Okamoto

doi:10.1007/BF02347732

Characterization of an Extended-Spectrum β-Lactamase from Escherichia coli Mediated by a Plasmid

Matsuhisa Inoue, Aisaku Fuse, Keizo Yamaguchi, Harushige Kanno, Mitsuo Kaku, Takashi Inamatsu, Tsutomu Nagahama, Masato Yoshida, Masato Nonoyama, Ryoichi Okamoto

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The β-lactamase from Escherichia coli ML 4953 pKU56 was purified about 190-fold from the crude cell-free extract with an overall recovery of 17%. The molecular weight mediated by pKU56 was about 28,000, and the isoelectric point was about 9.4. This enzyme hydrolyzed cephaloridine, cephalothin, cefamandole, cefuroxime, and cefsulodin at high rate, but ampicillin, piperacillin, and carbenicillin moderately. Antisera against pKU56 enzyme showed partly cross-reaction with β-lactamase from Klebsiella oxytoca and Proteus vulgaris.

Original language	English
Pages (from-to)	70-72
Number of pages	3
Journal	Journal of Infection and Chemotherapy
Volume	1
Issue number	1
DOIs	https://doi.org/10.1007/BF02347732
Publication status	Published - 1995 Jan 1

Keywords

extended-spectrum
pKU56
β-lactamase

ASJC Scopus subject areas

Microbiology (medical)
Pharmacology (medical)
Infectious Diseases

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10.1007/BF02347732

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Characterization of an Extended-Spectrum β-Lactamase from Escherichia coli Mediated by a Plasmid. / Inoue, Matsuhisa; Fuse, Aisaku; Yamaguchi, Keizo; Kanno, Harushige; Kaku, Mitsuo; Inamatsu, Takashi; Nagahama, Tsutomu; Yoshida, Masato; Nonoyama, Masato; Okamoto, Ryoichi.

In: Journal of Infection and Chemotherapy, Vol. 1, No. 1, 01.01.1995, p. 70-72.

Research output: Contribution to journal › Article › peer-review

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AU - Kanno, Harushige

AU - Kaku, Mitsuo

AU - Inamatsu, Takashi

AU - Nagahama, Tsutomu

AU - Yoshida, Masato

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AU - Okamoto, Ryoichi

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