Characterization of a precursor T-cell line (THP-6) with rearranged T-cell receptor β chain gene

Masayoshi Minegishi, Shigeru Tsuchiya, Naoko Minegishi, Osamu Yoshie, Masataka Nakamura, Tasuke Konno

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A previously established human leukemia cell line, designated THP-6, was further characterized with respect to cell surface antigen expression and immunoglobulin(Ig) and T-cell receptor(TCR) gene status. THP-6 cells were positive for CD7 and CD5 antigens and terminal deoxynucleotidyl transferase, but negative for CD2, CD1, CD4, CD8, CD10, cytoplasmic and surface CD3 and HLA-DR antigens, suggesting a precursor T-cell line. Analysis of Ig and TCR β chain genes revealed that THP-6 had a rearranged TCR β chain gene and a germline Ig gene. These results, in agreement with its phenotype, confirmed that THP-6 was of the T-cell lineage.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalLeukemia Research
Volume12
Issue number3
DOIs
Publication statusPublished - 1988

Keywords

  • Precursor T cells
  • TCR β chain
  • gene rearrangement
  • leukemia cell line

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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