Characterization of a Neurospora crassa photolyase-deficient mutant generated by repeat induced point mutation of the phr gene

M. Shimura, Y. Ito, C. Ishii, H. Yajima, H. Linden, T. Harashima, A. Yasui, H. Inoue

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    25 Citations (Scopus)

    Abstract

    We produced a photolyase-deficient mutant by repeat induced point mutation using the Neurospora crassa photolyase gene cloned previously. This mutation identified a new gene, phr, which was mapped on the right arm of linkage group I by both RFLP mapping and conventional mapping. To investigate the relationship between photoreactivation and dark repair processes, especially excision repair, double mutants of phr with representative repair-defective mutants of different types were constructed and tested for UV sensitivity and photoreactivation. The results show that the phr mutation has no influence on dark repair. Tests with CPD and TC(6-4) photoproduct-specific antibodies demonstrated that the phr mutant is defective in CPD photolyase and confirmed that there is no TC(6-4) photolyase activity in N. crassa. Furthermore, N. crassa photolyase is not a blue light receptor in the signal transduction that induces carotenoid biosynthesis.

    Original languageEnglish
    Pages (from-to)12-20
    Number of pages9
    JournalFungal Genetics and Biology
    Volume28
    Issue number1
    DOIs
    Publication statusPublished - 1999 Oct

    Keywords

    • CPD photolyase
    • DNA repair
    • Neorospora crassa
    • Photoreactivation

    ASJC Scopus subject areas

    • Microbiology
    • Genetics

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