Characterization of a mouse recA-like gene specifically expressed in testis

Shusei Sato, Toshiyuki Kobayashi, Yasuo Hotta, Satoshi Tabata

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Using the sequence information on a class of read-like genes, which have been isolated from meiosis-specific cDNA libraries of Lilium and budding yeast and identified in various plant cells, a new gene was screened from a mouse testis cDNA library. The putative product of the gene, termed MmLim15, was 37.8 kDa consisting of 340 amino acid residues. The predicted amino acid sequence showed 72-78% similarity to those of Lim15 from Lilium and other meiosis-specific RecA-like proteins. Similarity was also found to another class of read-like proteins, Rad51 and its homologues, which have been detected in both budding and fission yeasts and various animal cells, but to a lesser extent and the similarity patterns were somewhat different from those for Lim15 type proteins, revealing that the two classes of read-like genes are phylogenetically separate. Reverse transcription-PCR using poly(A)+ RNAs from various tissues as templates indicated that transcription of MmLim15 occurred specifically in the testis, suggesting that the MmLim15 product participates in meiotic recombination.

Original languageEnglish
Pages (from-to)147-150
Number of pages4
JournalDNA Research
Volume2
Issue number3
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Keywords

  • DMC1
  • LIM15
  • Meiotic recombination
  • Mouse
  • RecA-like gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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