Abstract
Using the sequence information on a class of read-like genes, which have been isolated from meiosis-specific cDNA libraries of Lilium and budding yeast and identified in various plant cells, a new gene was screened from a mouse testis cDNA library. The putative product of the gene, termed MmLim15, was 37.8 kDa consisting of 340 amino acid residues. The predicted amino acid sequence showed 72-78% similarity to those of Lim15 from Lilium and other meiosis-specific RecA-like proteins. Similarity was also found to another class of read-like proteins, Rad51 and its homologues, which have been detected in both budding and fission yeasts and various animal cells, but to a lesser extent and the similarity patterns were somewhat different from those for Lim15 type proteins, revealing that the two classes of read-like genes are phylogenetically separate. Reverse transcription-PCR using poly(A)+ RNAs from various tissues as templates indicated that transcription of MmLim15 occurred specifically in the testis, suggesting that the MmLim15 product participates in meiotic recombination.
Original language | English |
---|---|
Pages (from-to) | 147-150 |
Number of pages | 4 |
Journal | DNA Research |
Volume | 2 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1995 |
Externally published | Yes |
Keywords
- DMC1
- LIM15
- Meiotic recombination
- Mouse
- RecA-like gene
ASJC Scopus subject areas
- Molecular Biology
- Genetics