Characterization of a class II defective transposon carrying two haloacetate dehalogenase genes from Delftia acidovorans plasmid pUO1

Masahiro Sota, Masahiro Endo, Keiji Nitta, Haruhiko Kawasaki, Masataka Tsuda

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    28 Citations (Scopus)

    Abstract

    The two haloacetate dehalogenase genes, dehH1 and dehH2, on the 65-kb plasmid pUO1 from Delftia acidovorans strain B were found to be located on transposable elements. The dehH2 gene was carried on an 8.9-kb class I composite transposon (TnHad1) that was flanked by two directly repeated copies of IS1071, IS1071L and IS1071R. The dehH1 gene was also flanked by IS1071L and a truncated version of IS1071 (IS1071N). TnHad1, dehH1, and IS1071N were located on a 15.6-kb class II transposon (TnHad2) whose terminal inverted repeats and res site showed high homology with those of the Tn21-related transposons. TnHad2 was defective in transposition because of its lacking the transposase and resolvase genes. TnHad2 could transpose when the Tn21-encoded transposase and resolvase were supplied in trans. These results demonstrated that TnHad2 is a defective Tn21-related transposon carrying another class I catabolic transposon.

    Original languageEnglish
    Pages (from-to)2307-2315
    Number of pages9
    JournalApplied and environmental microbiology
    Volume68
    Issue number5
    DOIs
    Publication statusPublished - 2002

    ASJC Scopus subject areas

    • Biotechnology
    • Food Science
    • Applied Microbiology and Biotechnology
    • Ecology

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