Characterization of 11-dehydro-thromboxane B2 recombinant antibody obtained by phage display technology

Lilian Rumi Tsuruta, Yoshihisa Tomioka, Takanori Hishinuma, Yoshinori Kato, Kunihiko Itoh, Toshio Suzuki, Hiroki Oguri, Masahiro Hirama, Junichi Goto, Michinao Mizugaki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Recombinant monoclonal antibodies specific for 11-dehydro-thromboxane B2 (11D-TX) were isolated from the combinatorial libraries on a pComb3 phage-display vector using a magnetic cell sorting (MACS) system. The libraries were constructed from repertories of light and heavy-chains derived from the total RNA of 11D-TX conjugated keyhole limpet haemocyanin-immunized mice. Biotinylation of 11D-TX conjugated bovine serum albumin (BSA) was performed through free thiol groups on BSA using 1-biotinamido-4-[4′-(maleimidomethyl) cyclohexanecarboxamido] butane (Biotin-BMCC). Affinity bio-panning was performed to enrich the phage display libraries against biotinylated 11D-TX conjugated BSA with the MACS system. Results indicated that the selected anti-11D-TX Fab fragments expressed by E. coli exhibited a five-fold higher affinity for BSA conjugated 11D-TX compared to BSA alone and little specificity to other related compounds as determined by the binding assay and inhibition enzyme-linked immunosorbent assay (ELISA). This is the first report of an antibody against prostaglandin produced by phage display technology and also determination of the DNA sequence of this antibody. The MACS system was shown to be a simpler and more efficient method of panning than the conventional ELISA procedure. According to our results, we concluded that the phage display technique combined with the MACS system allowed the selection of the antibody with high affinity and some specificity.

Original languageEnglish
Pages (from-to)273-284
Number of pages12
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume68
Issue number4
DOIs
Publication statusPublished - 2003 Apr 1

Keywords

  • MACS
  • Monoclonal antibody
  • Phage display
  • Thromboxane

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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