Characteristics of two genes encoding proteins with an ADAM-type metalloprotease domain, which are induced during the molting periods in Bombyx mori

Manabu Ote, Kazuei Mita, Hideki Kawasaki, Masahiko Kobayashi, Toru Shimada

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

By microarray analyses, we identified two genes (BmADAMTS-1 and BmADAMTS-like) encoding a protein, which are induced during the pupal ecdysis in the wing discs of Bombyx mori; these genes are homologous to ADAMTS family members (a disintegrin and metalloproteinase domain, with thrombospondin type-1 repeats). A complete metal-binding motif of the ADAM-type metalloprotease domain (HEXXHXXGXXHD) was contained in both amino acid sequences. However, thrombospondin type 1 (TSP-1) repeats were observed only in BmADAMTS-1. The BmADAMTS-1 gene was expressed in the hemocyte and midgut of the larvae at day 2 of wandering stage (W2), and strongly induced during the pupal ecdysis in the hemolymph. The BmADAMTS-like gene was expressed in the epithelial tissues of the larvae at W2, and had expression peaks slightly later than the BmADAMTS-1 gene. Our results indicate that BmADAMTS-1 and BmADAMTS-like may cleave the extracellular matrix (ECM) in the degenerating and remodeling tissues during the molting periods.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalArchives of Insect Biochemistry and Physiology
Volume59
Issue number2
DOIs
Publication statusPublished - 2005 Jun 1

Keywords

  • Bombyx mori
  • Extracellular matrix
  • Metalloproteinase
  • Metamorphosis

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

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