Characteristics of the biotin enhancement of glucose-induced insulin release in pancreatic islets of the rat

Hideyuki Sone, Michiko Ito, Muneshige Shimizu, Yuka Sasaki, Michio Komai, Yuji Furukawa

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Perifused isolated rat islets were used to show that biotin plus 16.5 mM glucose evoked more insulin secretion than 16.5 mM glucose alone. Whether or not this reinforcement of glucose-induced insulin secretion by biotin is unique was studied by using perifused islets stimulated with 16.5 mM glucose plus 100 μM of one of various components of the vitamin B group. No effect of any of these vitamins was found on glucose-induced insulin secretion. These results indicate that biotin is unique among the members of the vitamin B group in enhancing glucose-induced insulin secretion. Static incubation experiments showed that biotin did not potentiate insulin release when the islets were incubated with an experimental solution containing either no or 2.8 mM glucose. The addition of biotin to 27.7 mM glucose, which is the maximal concentration for stimulating insulin release, did not significantly enhance the effect of the glucose on insulin release (although it did at 16.5 mM glucose). These findings indicate that biotin, by itself, does not stimulate insulin secretion, and does not enhance glucose-induced insulin secretion beyond the ability of glucose itself to stimulate insulin secretion.

Original languageEnglish
Pages (from-to)550-554
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Issue number3
Publication statusPublished - 2000 Jan 1


  • Biotin
  • Insulin release
  • Pancreatic islet
  • Vitamin B group

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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