Characteristics of nanogel-DNA complexes

Shin Ichi Sawada, Naomi Miyazawa, Shin Ichiro M. Nomura, Kazunari Akiyoshi

Research output: Contribution to conferencePaperpeer-review

1 Citation (Scopus)

Abstract

We reported that hydrogel nanoparticles (nanogels) were formed by the self-assembly of cholesterol group bearing pullulans (CHP). We synthesized amino group bearing CHP (CHP-NH2). CHP-NH2 formed monodisperse cationic nanogels (∼20 nm) in water. CHP-NH2 nanogels formed complexes with DNAs. In this study, we investigated the interaction between CHP-NH2 nanogels-plasmid DNA complexes and cells. We selected the plasmid DNA to express the green fluorescent protein (GFP) in mammalian cell, for use as a transfection marker. The cells that were incubated with CHP-NH2 nanogels-plasmid DNA complexes expressed GFP. This result indicates that CHP-NH2 nanogel could be used as efficient DNA carrier.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Externally publishedYes
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Keywords

  • CHP
  • DNA
  • Gene transfection
  • Nanogel

ASJC Scopus subject areas

  • Engineering(all)

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