Characteristics of aluminum-phosphate-adapted carrot cells: Uptake and utilization of the phosphate

Hiroyuki Koyama, Kunihiko Ojima, Tomoyuki Yamaya

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    15 Citations (Scopus)

    Abstract

    To elucidate the mechanism responsible for the superior growth of a selected line of carrot cells (Daucus carota L. cv MS Yonsun) in medium that contained AIPO4, kinetic studies of the uptake of phosphate and the efficiency of utilization of phosphate were performed with the selected cells and the wild-type cells. When the two cell lines were grown in a medium with adequate soluble phosphate (2 mM), there was no difference between their growth rates. Rates of increase in fresh weight as a function of increasing concentration of phosphate in the medium were also identical between the cell lines, indicating that the efficiency of utilization of phosphate by the selected cell line was similar to that by the wild-type cells. However, rate of uptake of phosphate by the selected cells under phosphate limited conditions (20μM NaH2PO4 at pH 5.6) was about 5-fold higher than that by the wild-type cells. Apparent Km values for the uptake of phosphate were calculated to be 13.6 and 9.1 μM for the selected and the wild-type cells, respectively. The Vmax valuewas estimated to be 88.8 nmol per g fresh weight per min for the selected cells and 28.2 for thewild-type cells. Thus, the selected cells has an enhanced system for uptake of phosphate wherebythere was an increase in the rate of the uptake without any dramatic change in the affinity for phosphateions.

    Original languageEnglish
    Pages (from-to)171-176
    Number of pages6
    JournalPlant and Cell Physiology
    Volume33
    Issue number2
    Publication statusPublished - 1992 Mar 1

    Keywords

    • Aluminum phosphate
    • Carrot cell
    • Daucus carota
    • Phosphate uptake
    • Stress selection

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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