TY - JOUR
T1 - Chaperonin-mediated stabilization and ATP-triggered release of semiconductor nanoparticles
AU - Ishii, Daisuke
AU - Kinbara, Kazushi
AU - Ishida, Yasuhiro
AU - Ishii, Noriyuki
AU - Okochi, Mina
AU - Yohda, Masafumi
AU - Aida, Takuzo
N1 - Funding Information:
Acknowledgements We thank K. Konishi for his initial contribution to the present work; K. Tsumoto for discussions; J. Oono and M. Nakamura for SEC analysis with MALS. N.I. was responsible for TEM microscopy. We acknowledge support from the 21st Century COE Programs of Research and Education (T.A., Human–Friendly Materials Based on Chemistry; M.Y., Future Nano-Materials), and from the JST ERATO Nanospace program. K.K. acknowledges support from the Nissan Science Foundation.
Funding Information:
Acknowledgements We thank S. Limpijumnong, M. Fuchs, M. Chabinyc, D. Biegelsen and the late J. McCaldin for discussions and support. This work was supported in part by the Air Force Office of Scientific Research and by the Deutsche Forschungsgemeinschaft.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/6/5
Y1 - 2003/6/5
N2 - Various properties of semiconductor nanoparticles, including photoluminescence and catalytic activity, make these materials attractive for a range of applications. As nanoparticles readily coagulate and so lose their size-dependent properties, shape-persistent three-dimensional stabilizers that enfold nanoparticles have been exploited. However, such wrapping approaches also make the nanoparticles insensitive to external stimuli, and so may limit their application. The chaperonin proteins GroEL (from Escherichia coli) and T.th ('T.th cpn', from Thermus thermophilus HB8) encapsulate denatured proteins inside a cylindrical cavity; after refolding, the encapsulated proteins are released by the action of ATP inducing a conformational change of the cavity. Here we report that GroEL and T.th cpn can also enfold CdS semiconductor nanoparticles, giving them high thermal and chemical stability in aqueous media. Analogous to the biological function of the chaperonins, the nanoparticles can be readily released from the protein cavities by the action of ATP. We expect that integration of such biological mechanisms into materials science will open a door to conceptually new bio-responsive devices.
AB - Various properties of semiconductor nanoparticles, including photoluminescence and catalytic activity, make these materials attractive for a range of applications. As nanoparticles readily coagulate and so lose their size-dependent properties, shape-persistent three-dimensional stabilizers that enfold nanoparticles have been exploited. However, such wrapping approaches also make the nanoparticles insensitive to external stimuli, and so may limit their application. The chaperonin proteins GroEL (from Escherichia coli) and T.th ('T.th cpn', from Thermus thermophilus HB8) encapsulate denatured proteins inside a cylindrical cavity; after refolding, the encapsulated proteins are released by the action of ATP inducing a conformational change of the cavity. Here we report that GroEL and T.th cpn can also enfold CdS semiconductor nanoparticles, giving them high thermal and chemical stability in aqueous media. Analogous to the biological function of the chaperonins, the nanoparticles can be readily released from the protein cavities by the action of ATP. We expect that integration of such biological mechanisms into materials science will open a door to conceptually new bio-responsive devices.
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U2 - 10.1038/nature01663
DO - 10.1038/nature01663
M3 - Article
C2 - 12789335
AN - SCOPUS:0037497251
VL - 423
SP - 628
EP - 632
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6940
ER -