Abstract
Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. Treatment of the MDV-derived lymphoblastoid cell lines MDCC-MSB1 (MSB1) and MDCC-RP1 (RP1) with 5-azacytidine (5-AzC) results in hypomethylation of MDV DNA. An increase in mRNA from certain portions of MDV DNA, including the BamHI-H region, was observed in 5-AzC-treated MSB1 cells, but not in the agent-treated RP1 cells. After the treatment of cells with 5-AzC, a site hypersensitive to digestion with DNaseI appeared in the BamHI-H region of MDV DNA in MSB1 but not in RP1. These results suggested that the enhancement of mRNA synthesis by 5-AzC is associated with changes in the nucleosomal structure of MDV DNA in lymphoblastoid cell line MSB1.
Original language | English |
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Pages (from-to) | 157-160 |
Number of pages | 4 |
Journal | Journal of Veterinary Medical Science |
Volume | 57 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1995 |
Keywords
- MDV DNA
- lymphoblastoid cell
- nucleosomal structure
ASJC Scopus subject areas
- veterinary(all)