Changes in the nucleosomal structure of the marek's disease virus genome in lymphoblatoid cell line MDCC-MSB1 induced by 5-azacytidine

Masanobu Hayashi; Io Kazuko; Tatsuya Furuichi; Shaomin Ren; Tomomasa Watanabe; Shigeo Namioka; Emiko Isogai

doi:10.1292/jvms.57.157

Changes in the nucleosomal structure of the marek's disease virus genome in lymphoblatoid cell line MDCC-MSB1 induced by 5-azacytidine

Masanobu Hayashi, Io Kazuko, Tatsuya Furuichi, Shaomin Ren, Tomomasa Watanabe, Shigeo Namioka, Emiko Isogai

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. Treatment of the MDV-derived lymphoblastoid cell lines MDCC-MSB1 (MSB1) and MDCC-RP1 (RP1) with 5-azacytidine (5-AzC) results in hypomethylation of MDV DNA. An increase in mRNA from certain portions of MDV DNA, including the BamHI-H region, was observed in 5-AzC-treated MSB1 cells, but not in the agent-treated RP1 cells. After the treatment of cells with 5-AzC, a site hypersensitive to digestion with DNaseI appeared in the BamHI-H region of MDV DNA in MSB1 but not in RP1. These results suggested that the enhancement of mRNA synthesis by 5-AzC is associated with changes in the nucleosomal structure of MDV DNA in lymphoblastoid cell line MSB1.

Original language	English
Pages (from-to)	157-160
Number of pages	4
Journal	Journal of Veterinary Medical Science
Volume	57
Issue number	1
DOIs	https://doi.org/10.1292/jvms.57.157
Publication status	Published - 1995

Keywords

MDV DNA
lymphoblastoid cell
nucleosomal structure

ASJC Scopus subject areas

veterinary(all)

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10.1292/jvms.57.157

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title = "Changes in the nucleosomal structure of the marek's disease virus genome in lymphoblatoid cell line MDCC-MSB1 induced by 5-azacytidine",

abstract = "Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. Treatment of the MDV-derived lymphoblastoid cell lines MDCC-MSB1 (MSB1) and MDCC-RP1 (RP1) with 5-azacytidine (5-AzC) results in hypomethylation of MDV DNA. An increase in mRNA from certain portions of MDV DNA, including the BamHI-H region, was observed in 5-AzC-treated MSB1 cells, but not in the agent-treated RP1 cells. After the treatment of cells with 5-AzC, a site hypersensitive to digestion with DNaseI appeared in the BamHI-H region of MDV DNA in MSB1 but not in RP1. These results suggested that the enhancement of mRNA synthesis by 5-AzC is associated with changes in the nucleosomal structure of MDV DNA in lymphoblastoid cell line MSB1.",

keywords = "MDV DNA, lymphoblastoid cell, nucleosomal structure",

author = "Masanobu Hayashi and Io Kazuko and Tatsuya Furuichi and Shaomin Ren and Tomomasa Watanabe and Shigeo Namioka and Emiko Isogai",

year = "1995",

doi = "10.1292/jvms.57.157",

language = "English",

volume = "57",

pages = "157--160",

journal = "Journal of Veterinary Medical Science",

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publisher = "Japanese Society of Veterinary Science",

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T1 - Changes in the nucleosomal structure of the marek's disease virus genome in lymphoblatoid cell line MDCC-MSB1 induced by 5-azacytidine

AU - Hayashi, Masanobu

AU - Kazuko, Io

AU - Furuichi, Tatsuya

AU - Ren, Shaomin

AU - Watanabe, Tomomasa

AU - Namioka, Shigeo

AU - Isogai, Emiko

PY - 1995

Y1 - 1995

N2 - Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. Treatment of the MDV-derived lymphoblastoid cell lines MDCC-MSB1 (MSB1) and MDCC-RP1 (RP1) with 5-azacytidine (5-AzC) results in hypomethylation of MDV DNA. An increase in mRNA from certain portions of MDV DNA, including the BamHI-H region, was observed in 5-AzC-treated MSB1 cells, but not in the agent-treated RP1 cells. After the treatment of cells with 5-AzC, a site hypersensitive to digestion with DNaseI appeared in the BamHI-H region of MDV DNA in MSB1 but not in RP1. These results suggested that the enhancement of mRNA synthesis by 5-AzC is associated with changes in the nucleosomal structure of MDV DNA in lymphoblastoid cell line MSB1.

AB - Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. Treatment of the MDV-derived lymphoblastoid cell lines MDCC-MSB1 (MSB1) and MDCC-RP1 (RP1) with 5-azacytidine (5-AzC) results in hypomethylation of MDV DNA. An increase in mRNA from certain portions of MDV DNA, including the BamHI-H region, was observed in 5-AzC-treated MSB1 cells, but not in the agent-treated RP1 cells. After the treatment of cells with 5-AzC, a site hypersensitive to digestion with DNaseI appeared in the BamHI-H region of MDV DNA in MSB1 but not in RP1. These results suggested that the enhancement of mRNA synthesis by 5-AzC is associated with changes in the nucleosomal structure of MDV DNA in lymphoblastoid cell line MSB1.

KW - MDV DNA

KW - lymphoblastoid cell

KW - nucleosomal structure

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Changes in the nucleosomal structure of the marek's disease virus genome in lymphoblatoid cell line MDCC-MSB1 induced by 5-azacytidine

Abstract

Keywords

ASJC Scopus subject areas

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