Changes in the nucleosomal structure of the marek's disease virus genome in lymphoblatoid cell line MDCC-MSB1 induced by 5-azacytidine

Masanobu Hayashi, Io Kazuko, Tatsuya Furuichi, Shaomin Ren, Tomomasa Watanabe, Shigeo Namioka, Emiko Isogai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. Treatment of the MDV-derived lymphoblastoid cell lines MDCC-MSB1 (MSB1) and MDCC-RP1 (RP1) with 5-azacytidine (5-AzC) results in hypomethylation of MDV DNA. An increase in mRNA from certain portions of MDV DNA, including the BamHI-H region, was observed in 5-AzC-treated MSB1 cells, but not in the agent-treated RP1 cells. After the treatment of cells with 5-AzC, a site hypersensitive to digestion with DNaseI appeared in the BamHI-H region of MDV DNA in MSB1 but not in RP1. These results suggested that the enhancement of mRNA synthesis by 5-AzC is associated with changes in the nucleosomal structure of MDV DNA in lymphoblastoid cell line MSB1.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalJournal of Veterinary Medical Science
Volume57
Issue number1
DOIs
Publication statusPublished - 1995

Keywords

  • MDV DNA
  • lymphoblastoid cell
  • nucleosomal structure

ASJC Scopus subject areas

  • veterinary(all)

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