Changes in the nucleosomal structure of the marek's disease virus genome in lymphoblatoid cell line MDCC-MSB1 induced by 5-azacytidine

Masanobu Hayashi, Io Kazuko, Tatsuya Furuichi, Shaomin Ren, Tomomasa Watanabe, Shigeo Namioka, Emiko Isogai

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. Treatment of the MDV-derived lymphoblastoid cell lines MDCC-MSB1 (MSB1) and MDCC-RP1 (RP1) with 5-azacytidine (5-AzC) results in hypomethylation of MDV DNA. An increase in mRNA from certain portions of MDV DNA, including the BamHI-H region, was observed in 5-AzC-treated MSB1 cells, but not in the agent-treated RP1 cells. After the treatment of cells with 5-AzC, a site hypersensitive to digestion with DNaseI appeared in the BamHI-H region of MDV DNA in MSB1 but not in RP1. These results suggested that the enhancement of mRNA synthesis by 5-AzC is associated with changes in the nucleosomal structure of MDV DNA in lymphoblastoid cell line MSB1.

    Original languageEnglish
    Pages (from-to)157-160
    Number of pages4
    JournalJournal of Veterinary Medical Science
    Volume57
    Issue number1
    DOIs
    Publication statusPublished - 1995

    Keywords

    • MDV DNA
    • lymphoblastoid cell
    • nucleosomal structure

    ASJC Scopus subject areas

    • veterinary(all)

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