TY - JOUR
T1 - Changes in the amounts of the NAD-dependent sorbitol dehydrogenase and its involvement in the development of apple fruit
AU - Yamaguchi, Hideaki
AU - Kanayama, Yoshinori
AU - Soejima, Junichi
AU - Yamaki, Shohei
PY - 1996/9
Y1 - 1996/9
N2 - Seasonal changes in the amounts of the NAD-dependent surbitol dehydrogenase (NAD-SDH) (enzyme code, 1.1.1.14) protein in developing apple (Malus pumila Mill var. domestica Schneid) fruit were determined by immunoblotting analysis. The amounts of the enzyme protein were very low in young fruit and rose as fruit matured. The weak correlation between enzyme protein and NAD-SDH activity and also the changes in NAD-SDH specific activity suggested that there could be posttranslational modification to the pre-existing enzyme or isoenzyme(s) of NAD-SDH. The changes in the amounts of NAD-SDH protein did not show the same pattern as those in relative growth rate, which is used to express sink activity, especially in young fruit. The role of NAD-SDH on sink activity in apple fruit, therefore, could not be explained simply by the amount and activity of the enzyme. In young fruit, it seems that enzymes other than NAD-SDH would be more directly related with fruit growth.
AB - Seasonal changes in the amounts of the NAD-dependent surbitol dehydrogenase (NAD-SDH) (enzyme code, 1.1.1.14) protein in developing apple (Malus pumila Mill var. domestica Schneid) fruit were determined by immunoblotting analysis. The amounts of the enzyme protein were very low in young fruit and rose as fruit matured. The weak correlation between enzyme protein and NAD-SDH activity and also the changes in NAD-SDH specific activity suggested that there could be posttranslational modification to the pre-existing enzyme or isoenzyme(s) of NAD-SDH. The changes in the amounts of NAD-SDH protein did not show the same pattern as those in relative growth rate, which is used to express sink activity, especially in young fruit. The role of NAD-SDH on sink activity in apple fruit, therefore, could not be explained simply by the amount and activity of the enzyme. In young fruit, it seems that enzymes other than NAD-SDH would be more directly related with fruit growth.
KW - Malus domestica
KW - relative growth rate
KW - sink activity
KW - sorbitol metabolism
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U2 - 10.21273/jashs.121.5.848
DO - 10.21273/jashs.121.5.848
M3 - Article
AN - SCOPUS:0030456443
VL - 121
SP - 848
EP - 852
JO - Journal of the American Society for Horticultural Science
JF - Journal of the American Society for Horticultural Science
SN - 0003-1062
IS - 5
ER -