Changes in the amounts of the NAD-dependent sorbitol dehydrogenase and its involvement in the development of apple fruit

Hideaki Yamaguchi, Yoshinori Kanayama, Junichi Soejima, Shohei Yamaki

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Seasonal changes in the amounts of the NAD-dependent surbitol dehydrogenase (NAD-SDH) (enzyme code, 1.1.1.14) protein in developing apple (Malus pumila Mill var. domestica Schneid) fruit were determined by immunoblotting analysis. The amounts of the enzyme protein were very low in young fruit and rose as fruit matured. The weak correlation between enzyme protein and NAD-SDH activity and also the changes in NAD-SDH specific activity suggested that there could be posttranslational modification to the pre-existing enzyme or isoenzyme(s) of NAD-SDH. The changes in the amounts of NAD-SDH protein did not show the same pattern as those in relative growth rate, which is used to express sink activity, especially in young fruit. The role of NAD-SDH on sink activity in apple fruit, therefore, could not be explained simply by the amount and activity of the enzyme. In young fruit, it seems that enzymes other than NAD-SDH would be more directly related with fruit growth.

Original languageEnglish
Pages (from-to)848-852
Number of pages5
JournalJournal of the American Society for Horticultural Science
Volume121
Issue number5
Publication statusPublished - 1996 Sep 1
Externally publishedYes

Keywords

  • Malus domestica
  • relative growth rate
  • sink activity
  • sorbitol metabolism

ASJC Scopus subject areas

  • Genetics
  • Horticulture

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