Ceramide enhances susceptibility of membrane phospholipids to phospholipase A2 through modification of lipid organization in platelet membranes

Tsutomu Hashizume, Kazuyuki Kitatani, Tadashi Kageura, Misako Hayama, Satoshi Akiba, Takashi Sato

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The effects of ceramide on agonist-stimulated phospholipase A2 (PLA2) activity were studied in platelets. Cell-permeable C6-ceramide (N- hexanoylsphingosine) exogenously added to platelet suspension enhanced U46619-stimulated arachidonic acid release and lysophosphatidylcholine production. Treatment of platelets with sphingomyelinase also led to an enhancement of the release. The enhanced arachidonic acid release by exogenous ceramide was completely inhibited by methyl arachidonyl fluorophosphonate, a cytosolic PLA2 inhibitor. However, U46619-stimulated PLA2 activity was not significantly potentiated by ceramide. These results suggest that enrichment of ceramide in membranes causes modification of intermolecular organization, leading to increased susceptibility of substrate phospholipids to PLA2.

Original languageEnglish
Pages (from-to)1275-1278
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume22
Issue number12
DOIs
Publication statusPublished - 1999 Dec

Keywords

  • Ceramide
  • Membrane perturbation
  • Phospholipase A
  • Platelet
  • U46619

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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