Centromere dynamics in the primitive red alga Cyanidioschyzon merolae

Shinichiro Maruyama, Haruko Kuroiwa, Shin Ya Miyagishima, Kan Tanaka, Tsuneyoshi Kuroiwa

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Centromeres are universally conserved functional units in eukaryotic linear chromosomes, but little is known about the structure and dynamics of the centromere in lower photosynthetic eukaryotes. Here we report the identification of a centromere marker protein CENH3 and visualization of centromere dynamics in the ultra-small primitive red alga Cyanidioschyzon merolae. Immunoblotting and immunofluorescence microscopy showed that CENH3 increased rapidly during S phase, followed by a drastic reconstitution into two discrete foci adjacent to the spindle poles at metaphase, suggesting the cell-cycle-regulated expression of CENH3. Immunoelectron microscopy revealed that the CENH3 signals were associated with the nuclear envelope, implying interplay between the kinetochore complex and the nuclear envelope. These results demonstrate dynamic centromere reconstitution during the cell cycle in an organism in which the chromosomes do not condense at metaphase.

Original languageEnglish
Pages (from-to)1122-1129
Number of pages8
JournalPlant Journal
Volume49
Issue number6
DOIs
Publication statusPublished - 2007 Mar

Keywords

  • CENH3
  • Centromere
  • Chromosome segregation
  • Cyanidioschyzon merolae

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Centromere dynamics in the primitive red alga Cyanidioschyzon merolae'. Together they form a unique fingerprint.

Cite this