Centrin is essential for the activity of the ciliary reversal-coupled voltage-gated Ca 2+ channels

Kohsuke Gonda, Akiko Yoshida, Kazunori Oami, Mihoko Takahashi

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Voltage-gated Ca 2+ channels play a critical role in controlling Ca 2+ entry in various cells. Ciliary reversal in Paramecium depends on the Ca 2+ influx through voltage-gated Ca 2+ channels on the ciliary membrane. One of the voltage-gated Ca 2+ channel mutants in Paramecium caudatum, cnrC, neither produces Ca 2+ action potentials nor responds to any depolarizing stimuli. Here, we report that the cnrC + gene product is P. caudatum centrin (Pccentrin1p), a member of the Ca 2+-binding EF-hand protein superfamily. The Pccentrin1p gene of cnrC was found to contain a single-base deletion, a mutation that caused the loss of the fourth EF-hand of Pccentrin1p. Moreover, the wild-type Ca 2+ channel function was impaired by Pccentrin1p gene silencing, leading to the loss of current-evoked Ca 2+ action potentials and stimulated ciliary reversal. These results demonstrate that Pccentrin1p is indispensable for the activity of the voltage-gated Ca 2+ channels that control ciliary reversal in Paramecium.

Original languageEnglish
Pages (from-to)891-897
Number of pages7
JournalBiochemical and biophysical research communications
Volume323
Issue number3
DOIs
Publication statusPublished - 2004 Oct 22
Externally publishedYes

Keywords

  • Action potential
  • Behavioral mutant
  • Ca -binding protein
  • Centrin
  • Ciliary reversal
  • EF-hand
  • Paramecium
  • RNAi
  • Voltage-gated Ca channel
  • cnrC

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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