Abstract
Cellulase (EC 3.2.1.4) can be used for the enzyme-catalysed synthesis of oligosaccharides in a mixed buffer/organic solvent system. Besides glucopyranosides and cellooligosaccharides, non-glucosidic saccharides and modified glucoses as glycosyl acceptors also react with β-lactosyl fluoride as glycosyl donor. The reaction proceeds in a completely stereo- and regio-specific manner, giving rise to coupling products having β(1→4) glycosidic bonds exclusively. Based on these results, a model for the active site of cellulase (Trichoderma viride) and the orientation of the donor and acceptor therein is proposed.
| Original language | English |
|---|---|
| Pages (from-to) | 1851-1857 |
| Number of pages | 7 |
| Journal | Journal of the Chemical Society, Perkin Transactions 1 |
| Issue number | 13 |
| Publication status | Published - 1994 Dec 1 |
ASJC Scopus subject areas
- Chemistry(all)
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Karthaus, O., Shoda, S., Takano, H., Obata, K., & Kobayashi, S. (1994). Cellulase-catalysed glycosylation reactions: Simple route towards a highly selective synthesis of oligosaccharides. Journal of the Chemical Society, Perkin Transactions 1, (13), 1851-1857.