Cellulase-catalysed glycosylation reactions: Simple route towards a highly selective synthesis of oligosaccharides

Olaf Karthaus, Shinichiro Shoda, Hiroshi Takano, Kei Obata, Shiro Kobayashi

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Cellulase (EC 3.2.1.4) can be used for the enzyme-catalysed synthesis of oligosaccharides in a mixed buffer/organic solvent system. Besides glucopyranosides and cellooligosaccharides, non-glucosidic saccharides and modified glucoses as glycosyl acceptors also react with β-lactosyl fluoride as glycosyl donor. The reaction proceeds in a completely stereo- and regio-specific manner, giving rise to coupling products having β(1→4) glycosidic bonds exclusively. Based on these results, a model for the active site of cellulase (Trichoderma viride) and the orientation of the donor and acceptor therein is proposed.

Original languageEnglish
Pages (from-to)1851-1857
Number of pages7
JournalJournal of the Chemical Society, Perkin Transactions 1
Issue number13
Publication statusPublished - 1994 Dec 1

ASJC Scopus subject areas

  • Chemistry(all)

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