Abstract
Expression, purification, and characterization of single-species of membrane protein are one of the most important technologies in post-genome era. Especially membrane proteins are difficult to isolate and purify with keeping their function. We engaged to study direct reconstitution of cell-free synthesized membrane proteins to giant liposomes. Recently we reported that the spontaneous formation of the three-dimensional lipid tubular network in the presence of various gangliosides or cholesterol. Here we report the experimental trial to obtain the fixed liposome-network with functional membrane proteins. As a model membrane protein, cytochrome-b5 was adopted. In the presence of liposomes, the expressed membrane proteins were directly and effectively displayed on the liposomal surface without aggregation. The proteo-liposomes were fixed to tubular-connected networks by adding gangliosides. The network formation process in the agarose gel was directly observed by fluorescent microscopy. Hemispheric liposomes changed their form to the tubular-networks in the gelation process. The stable lipid nanotube network can be used for constructing a biochemical micro-reactor to analyze membrane protein functions.
Original language | English |
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Pages | 5231-5232 |
Number of pages | 2 |
Publication status | Published - 2006 Dec 1 |
Externally published | Yes |
Event | 55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: 2006 Sep 20 → 2006 Sep 22 |
Other
Other | 55th Society of Polymer Science Japan Symposium on Macromolecules |
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Country | Japan |
City | Toyama |
Period | 06/9/20 → 06/9/22 |
Keywords
- Giant liposome
- Membrane protein
- Micro reactor
- Nanotube network
- Self-organization
ASJC Scopus subject areas
- Engineering(all)