Cellularmembrane-like structure of tubular-linked liposome network

Shin Ichiro M. Nomura, Kazunari Akiyoshi

Research output: Contribution to conferencePaperpeer-review

Abstract

Expression, purification, and characterization of single-species of membrane protein are one of the most important technologies in post-genome era. Especially membrane proteins are difficult to isolate and purify with keeping their function. We engaged to study direct reconstitution of cell-free synthesized membrane proteins to giant liposomes. Recently we reported that the spontaneous formation of the three-dimensional lipid tubular network in the presence of various gangliosides or cholesterol. Here we report the experimental trial to obtain the fixed liposome-network with functional membrane proteins. As a model membrane protein, cytochrome-b5 was adopted. In the presence of liposomes, the expressed membrane proteins were directly and effectively displayed on the liposomal surface without aggregation. The proteo-liposomes were fixed to tubular-connected networks by adding gangliosides. The network formation process in the agarose gel was directly observed by fluorescent microscopy. Hemispheric liposomes changed their form to the tubular-networks in the gelation process. The stable lipid nanotube network can be used for constructing a biochemical micro-reactor to analyze membrane protein functions.

Original languageEnglish
Pages5231-5232
Number of pages2
Publication statusPublished - 2006 Dec 1
Externally publishedYes
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: 2006 Sep 202006 Sep 22

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period06/9/2006/9/22

Keywords

  • Giant liposome
  • Membrane protein
  • Micro reactor
  • Nanotube network
  • Self-organization

ASJC Scopus subject areas

  • Engineering(all)

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