Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells

Lukasz Langowski, Krzysztof Wabnik, Hongjiang Li, Steffen Vanneste, Satoshi Naramoto, Hirokazu Tanaka, Jiri Friml

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    The asymmetric localization of proteins in the plasma membrane domains of eukaryotic cells is a fundamental manifestation of cell polarity that is central to multicellular organization and developmental patterning. In plants, the mechanisms underlying the polar localization of cargo proteins are still largely unknown and appear to be fundamentally distinct from those operating in mammals. Here, we present a systematic, quantitative comparative analysis of the polar delivery and subcellular localization of proteins that characterize distinct polar plasma membrane domains in plant cells. The combination of microscopic analyses and computational modeling revealed a mechanistic framework common to diverse polar cargos and underlying the establishment and maintenance of apical, basal, and lateral polar domains in plant cells. This mechanism depends on the polar secretion, constitutive endocytic recycling, and restricted lateral diffusion of cargos within the plasma membrane. Moreover, our observations suggest that polar cargo distribution involves the individual protein potential to form clusters within the plasma membrane and interact with the extracellular matrix. Our observations provide insights into the shared cellular mechanisms of polar cargo delivery and polarity maintenance in plant cells.

    Original languageEnglish
    Article number16018
    JournalCell Discovery
    Volume2
    DOIs
    Publication statusPublished - 2016 Jul 19

    Keywords

    • Protein trafficking
    • lateral diffusion
    • polar recycling
    • polar secretion
    • protein clustering
    • protein dynamics modeling

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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