Cellular conservation of endangered midget buffalo (Lowland Anoa, Bubalus quarlesi) by establishment of primary cultured cell, and its immortalization with expression of cell cycle regulators

Tomokazu Fukuda, Yuuka Iino, Takahiro Eitsuka, Manabu Onuma, Masafumi Katayama, Koichi Murata, Miho Inoue-Murayama, Kumiko Hara, Emiko Isogai, Tohru Kiyono

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Lowland Anoa has become endangered due to hunting and human activity. Protection and breeding of endangered species in a controlled environment is the best way of conservation. However, it is not possible to adopt this approach for all endangered species because of the cost involved and the ever-increasing number of critically endangered species. In consideration of these limitations to the conventional conservation methods, we established a primary cell culture of endangered buffalo (Lowland Anoa, Bubalus quarlesi), for the preservation of this biological resource. In addition, we introduced human derived, mutant cyclin dependent kinase 4 (CDK4), Cyclin D, and telomerase reverse transcriptase (TERT) into the primary cells. The successful introduction of these three genes was confirmed by western blot with specific antibodies, and enzymatic activity. We also showed that the expression of mutant CDK4, Cyclin D, and TERT allows us to efficiently establish an immortalized cell line, with an intact chromosome pattern, from Lowland Anoa. To the best of our knowledge, this study is the first investigation that established an immortalized cell line of an endangered wild animal species.

Original languageEnglish
Pages (from-to)1937-1947
Number of pages11
JournalCytotechnology
Volume68
Issue number5
DOIs
Publication statusPublished - 2016 Oct 1

Keywords

  • Cellular conservation
  • Cultured cells
  • Endangered animals
  • Immortalization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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