Cellular basis of the role of diesel exhaust particles in inducing Th2-dominant response

Tomoyuki Ohtani, Satoshi Nakagawa, Masahiro Kurosawa, Masato Mizuashi, Maki Ozawa, Setsuya Aiba

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

There is growing evidence that diesel exhaust particles (DEP) can induce allergic diseases with increased IgE production and preferential activation of Th2 cells. To clarify the cellular basis of the role of DEP in the induction of Th2-dominant responses, we examined the effects of DEP on the cytokine production by T cells stimulated with anti-CD3/CD28 Ab and on that by monocyte-derived dendritic cells (MoDCs) stimulated with CD40L and/or IFN-γ. We examined IFN-γ, IL-4, IL-5, IL-8, and IL-10 produced by T cells and TNF-α, IL-1β, IL-10, and IL-12 produced by MoDCs using real-time PCR analysis or by ELISA. To highlight the effects of DEP, we compared the effects of DEP with those of dexamethasone (DEX) and cyclosporin A (CyA). DEP significantly suppressed IFN-γ mRNA expression and protein production, while it did not affect IL-4 or IL-5 mRNA expression or protein production. The suppressive effect on IFN-γ mRNA expression was more potent than that of DEX and comparable at 30 μg/ml with 10-7 M CyA. The suppressive effect on IFN-γ production was also more potent than that of either DEX or CyA. DEP suppressed IL-12p40 and IL-12p35 HiRNA expression and IL-12p40 and IL-12p70 production by MoDCs, while it augmented IL-1β mRNA expression. Finally, by using a thiol antioxidant, N-acetyl cysteine, we found that the suppression of IFN-γ production by DEP-treated T cells was mediated by oxidative stress. These data revealed a unique characteristic of DEP, namely that they induce a Th2 cytokine milieu in both T cells and dendritic cells.

Original languageEnglish
Pages (from-to)2412-2419
Number of pages8
JournalJournal of Immunology
Volume174
Issue number4
DOIs
Publication statusPublished - 2005 Feb 15

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Cellular basis of the role of diesel exhaust particles in inducing Th2-dominant response'. Together they form a unique fingerprint.

Cite this