Cellular attachment behavior on biodegradable polymer surface immobilizing endothelial cell-specific peptide

Yuichi Ohya, Kazuki Nishimura, Hiromichi Sumida, Yuta Yoshizaki, Akinori Kuzuya, Atsushi Mahara, Tetsuji Yamaoka

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Small-caliber artificial blood vessels with inner diameters of smaller than 4 mm have not been put into practical use because of early thrombus formation and graft occlusion. To realize small-caliber artificial blood vessels with anti-thrombus property and long-term patency, one of the promising approaches is endothelialization of the lumen by tissue engineering approaches. Integrin α4β1 on the endothelial cell membrane is known to act as a receptor for Arg-Glu-Asp-Val (REDV) tetra-peptide, and this peptide can be used as a specific ligand to introduce endothelial cell attachment onto the surfaces of polymer scaffold. In this study, biodegradable polymer surface immobilizing REDV peptide were prepared, and the specific attachment of endothelial cells on it was investigated as a preliminary study for tissue-engineered small-caliber blood vessels in a future application. We synthesized copolymer of ε-caprolactone and depsipeptide having reactive carboxylic acid side-chain groups (PGDCL), and REDV peptide was attached to the copolymer to give PGDCL-REDV. The attachment of human umbilical vein endothelial cells (HUVECs) were investigated for the blend polymer film prepared by mixing PGDCL and PGDCL-REDV. The obtained blend polymer films exhibited sequence- and cell-specific HUVECs attachment through REDV peptide recognition. This technique should be useful not only to obtain artificial blood vessels which induce endothelialization and but also to provide biodegradable scaffolds with specific ligands immobilized surfaces for tissue regeneration.

Original languageEnglish
Pages (from-to)1475-1488
Number of pages14
JournalJournal of Biomaterials Science, Polymer Edition
Volume31
Issue number11
DOIs
Publication statusPublished - 2020 Jul 23
Externally publishedYes

Keywords

  • Biodegradable polymers
  • endothelial cells attachment
  • polydepsipeptide
  • regenerative blood vessels
  • tissue engineering

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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