Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub-zero acclimation

Daisuke Takahashi, Kim L. Johnson, Pengfei Hao, Tan Tuong, Alexander Erban, Arun Sampathkumar, Antony Bacic, David P. Livingston, Joachim Kopka, Takeshi Kuroha, Ryusuke Yokoyama, Kazuhiko Nishitani, Ellen Zuther, Dirk K. Hincha

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze–thaw cycle. Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation. In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation). Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation. In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH). The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure. Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant. Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.

Original languageEnglish
Pages (from-to)915-930
Number of pages16
JournalPlant Cell and Environment
Volume44
Issue number3
DOIs
Publication statusPublished - 2021 Mar
Externally publishedYes

Keywords

  • Abiotic stress
  • Arabidopsis thaliana
  • apoplast
  • cell wall remodeling
  • extracellular matrix
  • immunohistochemistry
  • xyloglucan

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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