Cell viability score as an integrated indicator for cytotoxicity of benzalkonium chloride-containing antiglaucoma eyedrops

Masahiko Ayaki, Atsuo Iwasawa, Yoshimi Niwano

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

We evaluated the in vitro cytotoxicity of benzalkonium chloride (BAK)-containing antiglaucoma eyedrops. We prepared cell cultures of SIRC, BCE C/D-1b, RC-1, and Chang conjunctiva. The viability of cell cultures was determined using the MTT and neutral red assays. The cell viability score (CVS) was used to compare the toxicity of test solutions. %CVS50 and %CVS40/80 of each eyedrop solution were 71 and 26 for Lumigan® (0.002% bimatoprost with 0.005% BAK), 100 and 99 for Tapros® (0.0015% tafluprost, a new formula from 2010 with 0.001% BAK), 39 and -29 for 2% Trusopt® (2% dorzolamide with 0.0075% BAK), 28 and -43 for Xalacom® (latanoprost/0.5% timolol with 0.02% BAK), 88 and 66 for DuoTrav® (travoprost/0.5% timolol with no BAK), 36 and -35 for Cosopt® (2% dorzolamide/0.5% timolol with 0.0075% BAK) and 53 and -1 for Combigan® (0.15% brimonidin/0.5% timolol with 0.005% BAK). Only Xalacom® and Tapros® did not show an apparent decrease in %CVS as compared to the corresponding concentration of BAK. In conclusion, the cytotoxicity of tested eyedrops was dependent on BAK. Only the eyedrops containing latanoprost or tafluprost showed a reduction in the cytotoxicity of BAK.

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalBiocontrol Science
Volume17
Issue number3
DOIs
Publication statusPublished - 2012

Keywords

  • Benzalkonium chloride
  • Cell viability score
  • Eye drops
  • Glaucoma
  • Toxicity

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health

Fingerprint Dive into the research topics of 'Cell viability score as an integrated indicator for cytotoxicity of benzalkonium chloride-containing antiglaucoma eyedrops'. Together they form a unique fingerprint.

Cite this