TY - JOUR
T1 - Cell type-specific transcriptome of brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing
AU - Osaka, Masaaki
AU - Matsuda, Tomoki
AU - Sakazono, Satomi
AU - Masuko-Suzuki, Hiromi
AU - Maeda, Shunsuke
AU - Sewaki, Misato
AU - Sone, Mikako
AU - Takahashi, Hirokazu
AU - Nakazono, Mikio
AU - Iwano, Megumi
AU - Takayama, Seiji
AU - Shimizu, Kentaro K.
AU - Yano, Kentaro
AU - Lim, Yong Pyo
AU - Suzuki, Go
AU - Suwabe, Keita
AU - Watanabe, Masao
N1 - Funding Information:
This work was supported by the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) [Grants-in-Aid for Scientific Research on Innovative Areas (Nos. 23113001 and 23113006 to G.S., K.S. and M.W., and No. 24113518 to K.Y.)]; the Japan Society for Promotion of Science (JSPS) [a Grant-in-Aid for Scientific Research (C) (No. 25450515 to G.S.), Grant-in-Aid for Scientific Research (B) (No. 25292005 to K.S. and M.W.)]; Meiji University [Research Funding for Computational Software Supporting Program (to K.Y.)].
PY - 2013/11
Y1 - 2013/11
N2 - Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.
AB - Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.
KW - Arabidopsis halleri
KW - Arabidopsis thaliana
KW - Brassica rapa
KW - Laser microdissection
KW - Papilla cell
KW - RNA sequencing
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U2 - 10.1093/pcp/pct133
DO - 10.1093/pcp/pct133
M3 - Article
C2 - 24058146
AN - SCOPUS:84887950958
VL - 54
SP - 1894
EP - 1906
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 11
ER -