Cell type-specific transcriptome of brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing

Masaaki Osaka, Tomoki Matsuda, Satomi Sakazono, Hiromi Masuko-Suzuki, Shunsuke Maeda, Misato Sewaki, Mikako Sone, Hirokazu Takahashi, Mikio Nakazono, Megumi Iwano, Seiji Takayama, Kentaro K. Shimizu, Kentaro Yano, Yong Pyo Lim, Go Suzuki, Keita Suwabe, Masao Watanabe

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

    Original languageEnglish
    Pages (from-to)1894-1906
    Number of pages13
    JournalPlant and Cell Physiology
    Volume54
    Issue number11
    DOIs
    Publication statusPublished - 2013 Nov

    Keywords

    • Arabidopsis halleri
    • Arabidopsis thaliana
    • Brassica rapa
    • Laser microdissection
    • Papilla cell
    • RNA sequencing

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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