Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium

Shun Shibata, Ryuhei Hayashi, Yuji Kudo, Toru Okubo, Tsutomu Imaizumi, Tomohiko Katayama, Yuki Ishikawa, Yuki Kobayashi, Junko Toga, Yukimasa Taniguchi, Yoichi Honma, Kiyotoshi Sekiguchi, Kohji Nishida

Research output: Contribution to journalArticle

Abstract

Transplantation of hiPSC-derived corneal epithelial cell (iCEC) sheets is expected to serve as a treatment for severe corneal diseases. The efficacy of this treatment depends on effective iCEC purification. Hayashi and colleagues used differential laminin adhesion of iCECs and non-CECs in combination magnetic cell sorting to develop a simple method to obtain highly pure iCEC sheets without using FACS.

Original languageEnglish
Pages (from-to)663-676
Number of pages14
JournalStem Cell Reports
Volume14
Issue number4
DOIs
Publication statusPublished - 2020 Apr 14
Externally publishedYes

Keywords

  • cell competition
  • cell purification
  • corneal epithelial cells (CECs)
  • human induced pluripotent stem cells (hiPSCs)
  • laminin isoforms
  • substrate-specific adhesion

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium'. Together they form a unique fingerprint.

  • Cite this

    Shibata, S., Hayashi, R., Kudo, Y., Okubo, T., Imaizumi, T., Katayama, T., Ishikawa, Y., Kobayashi, Y., Toga, J., Taniguchi, Y., Honma, Y., Sekiguchi, K., & Nishida, K. (2020). Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium. Stem Cell Reports, 14(4), 663-676. https://doi.org/10.1016/j.stemcr.2020.02.008