Cell type distinct accumulations of mRNA and protein for NADH-dependent glutamate synthase in rice roots in response to the supply of NH4+

Keiki Ishiyama, Soichi Kojima, Hideki Takahashi, Toshihiko Hayakawa, Tomoyuki Yamaya

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Transient and NH4+-inducible accumulation of the mRNA for NADH-dependent glutamate synthase (NADH-GOGAT; EC 1.4.1.14) in the roots of rice seedlings was analyzed in situ to identify the cell types responsible for the induction. The mRNA was detected specifically in sclerenchyma cells (the third cell-layer from the root surface), and the maximal accumulation was seen at 3-6 h following the supply of NH4+ ions. Expression of the NADH-GOGAT gene in sclerenchyma cells was also confirmed using transgenic rice plants expressing GUS reporter gene under the control of rice NADH-GOGAT promoter. On the other hand, clear signals for the NADH-GOGAT protein were detected in epidermial cells and exodermal cells (the first and second cell layers from the root surface) at 12 h, following the supply of NH4+ ions. The distinct localization of mRNA and protein for NADH-GOGAT suggests that either the mRNA or the translated protein in the sclerenchyma cells is migrated to the root surface. In contrast to NADH-GOGAT protein, Fd-GOGAT (EC 1.4.7.1) protein was detected in sclerenchyma cells, cortex cells, and stele in the rice roots. The distinct localization of the two GOGAT species indicates that they have different roles in the nitrogen metabolism in rice roots.

Original languageEnglish
Pages (from-to)643-647
Number of pages5
JournalPlant Physiology and Biochemistry
Volume41
Issue number6-7
DOIs
Publication statusPublished - 2003 Jun 1

Keywords

  • Cellular localization
  • NADH-dependent glutamate synthase (EC 1.4.1.14)
  • NH supply
  • Rice root

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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