Cell-to-cell movement of the CAPRICE protein in Arabidopsis root epidermal cell differentiation

Tetsuya Kurata, Tetsuya Ishida, Chie Kawabata-Awai, Masahiro Noguchi, Sayoko Hattori, Ryosuke Sano, Ryoko Nagasaka, Rumi Tominaga, Yoshihiro Koshino-Kimura, Tomohiko Kato, Shusei Sato, Satoshi Tabata, Kiyotaka Okada, Takuji Wada

    Research output: Contribution to journalArticlepeer-review

    194 Citations (Scopus)

    Abstract

    CAPRICE (CPC), a small, R3-type Myb-like protein, is a positive regulator of root hair development in Arabidopsis. Cell-to-cell movement of CPC is important for the differentiation of epidermal cells into trichoblasts (root hair cells). CPC is transported from atrichoblasts (hairless cells), where it is expressed, to trichoblasts, and generally accumulates in their nuclei. Using truncated versions of CPC fused to GFP, we identified a signal domain that is necessary and sufficient for CPC cell-to-cell movement. This domain includes the N-terminal region and a part of the Myb domain. Amino acid substitution experiments indicated that W76 and M78 in the Myb domain are critical for targeted transport, and that W76 is crucial for the nuclear accumulation of CPC:GFP. To evaluate the tissue-specificity of CPC movement, CPC:GFP was expressed in the stele using the SHR promoter and in trichoblasts using the EGL3 promoter. CPC:GFP was able to move from trichoblasts to atrichoblasts but could not exit from the stele, suggesting the involvement of tissue-specific regulatory factors in the intercellular movement of CPC. Analyses with a secretion inhibitor, Brefeldin A, and with an rhd3 mutant defective in the secretion process in root epidermis suggested that intercellular CPC movement is mediated through plasmodesmata. Furthermore, the fusion of CPC to tandem-GFPs defined the capability of CPC to increase the size exclusion limit of plasmodesmata.

    Original languageEnglish
    Pages (from-to)5387-5398
    Number of pages12
    JournalDevelopment
    Volume132
    Issue number24
    DOIs
    Publication statusPublished - 2005 Dec

    Keywords

    • Arabidopsis
    • CAPRICE
    • Epidermis
    • Myb
    • Protein movement

    ASJC Scopus subject areas

    • Molecular Biology
    • Developmental Biology

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