Cell-Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutant β-Lactamase-Tag Technology

Akimasa Yoshimura; Shin Mizukami; Yuichiro Hori; Shuji Watanabe; Kazuya Kikuchi

doi:10.1002/cbic.201100021

Cell-Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutant β-Lactamase-Tag Technology

Akimasa Yoshimura, Shin Mizukami, Yuichiro Hori, Shuji Watanabe, Kazuya Kikuchi

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Time-lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β-lactamase tag (BL-tag) technology for tethering target proteins and streptavidin-conjugated luminescent nanoparticles. This method enabled visualization of BL-tag-fused epidermal growth factor receptor (EGFR) internalization and pulse-chase labeling by using three kinds of quantum dots (QDs).

Original language	English
Pages (from-to)	1031-1034
Number of pages	4
Journal	ChemBioChem
Volume	12
Issue number	7
DOIs	https://doi.org/10.1002/cbic.201100021
Publication status	Published - 2011 May 2
Externally published	Yes

Keywords

Biotinylation
Epidermal growth factor receptor
Fluorescence
Mutant beta-lactamase
Quantum dots

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.201100021

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abstract = "Time-lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β-lactamase tag (BL-tag) technology for tethering target proteins and streptavidin-conjugated luminescent nanoparticles. This method enabled visualization of BL-tag-fused epidermal growth factor receptor (EGFR) internalization and pulse-chase labeling by using three kinds of quantum dots (QDs).",

keywords = "Biotinylation, Epidermal growth factor receptor, Fluorescence, Mutant beta-lactamase, Quantum dots",

author = "Akimasa Yoshimura and Shin Mizukami and Yuichiro Hori and Shuji Watanabe and Kazuya Kikuchi",

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language = "English",

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AU - Yoshimura, Akimasa

AU - Mizukami, Shin

AU - Hori, Yuichiro

AU - Watanabe, Shuji

AU - Kikuchi, Kazuya

PY - 2011/5/2

Y1 - 2011/5/2

N2 - Time-lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β-lactamase tag (BL-tag) technology for tethering target proteins and streptavidin-conjugated luminescent nanoparticles. This method enabled visualization of BL-tag-fused epidermal growth factor receptor (EGFR) internalization and pulse-chase labeling by using three kinds of quantum dots (QDs).

AB - Time-lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β-lactamase tag (BL-tag) technology for tethering target proteins and streptavidin-conjugated luminescent nanoparticles. This method enabled visualization of BL-tag-fused epidermal growth factor receptor (EGFR) internalization and pulse-chase labeling by using three kinds of quantum dots (QDs).

KW - Biotinylation

KW - Epidermal growth factor receptor

KW - Fluorescence

KW - Mutant beta-lactamase

KW - Quantum dots

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ER -

Cell-Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutant β-Lactamase-Tag Technology

Abstract

Keywords

ASJC Scopus subject areas

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