Cell-Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutant β-Lactamase-Tag Technology

Akimasa Yoshimura, Shin Mizukami, Yuichiro Hori, Shuji Watanabe, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Time-lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β-lactamase tag (BL-tag) technology for tethering target proteins and streptavidin-conjugated luminescent nanoparticles. This method enabled visualization of BL-tag-fused epidermal growth factor receptor (EGFR) internalization and pulse-chase labeling by using three kinds of quantum dots (QDs).

Original languageEnglish
Pages (from-to)1031-1034
Number of pages4
JournalChemBioChem
Volume12
Issue number7
DOIs
Publication statusPublished - 2011 May 2
Externally publishedYes

Keywords

  • Biotinylation
  • Epidermal growth factor receptor
  • Fluorescence
  • Mutant beta-lactamase
  • Quantum dots

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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