Cell plate restricted association of DRP1A and PIN proteins is required for cell polarity establishment in arabidopsis

Jozef Mravec, Jan Petrášek, Na Li, Sjef Boeren, Rumyana Karlova, Saeko Kitakura, Markéta Pařezová, Satoshi Naramoto, Tomasz Nodzyński, Pankaj Dhonukshe, Sebastian Y. Bednarek, Eva Zažímalová, Sacco De Vries, Jiří Friml

    Research output: Contribution to journalArticlepeer-review

    53 Citations (Scopus)

    Abstract

    The polarized transport of the phytohormone auxin [1], which is crucial for the regulation of different stages of plant development [2, 3], depends on the asymmetric plasma membrane distribution of the PIN-FORMED (PIN) auxin efflux carriers [4, 5]. The PIN polar localization results from clathrin-mediated endocytosis (CME) from the plasma membrane and subsequent polar recycling [6]. The Arabidopsis genome encodes two groups of dynamin-related proteins (DRPs) that show homology to mammalian dynamin - a protein required for fission of endocytic vesicles during CME [7, 8]. Here we show by coimmunoprecipitation (coIP), bimolecular fluorescence complementation (BiFC), and Förster resonance energy transfer (FRET) that members of the DRP1 group closely associate with PIN proteins at the cell plate. Localization and phenotypic analysis of novel drp1 mutants revealed a requirement for DRP1 function in correct PIN distribution and in auxin-mediated development. We propose that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins and the associated CME machinery from the cell plate membranes during cytokinesis is an important mechanism for proper polar PIN positioning in interphase cells.

    Original languageEnglish
    Pages (from-to)1055-1060
    Number of pages6
    JournalCurrent Biology
    Volume21
    Issue number12
    DOIs
    Publication statusPublished - 2011 Jun 21

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

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