TY - JOUR
T1 - Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells
AU - Okamura, Daiji
AU - Maeda, Ikuma
AU - Taniguchi, Hirofumi
AU - Tokitake, Yuko
AU - Ikeda, Makiko
AU - Ozato, Keiko
AU - Mise, Nathan
AU - Abe, Kuniya
AU - Noce, Toshiaki
AU - Belmonte, Juan Carlos Izpisua
AU - Matsui, Yasuhisa
PY - 2012/11/15
Y1 - 2012/11/15
N2 - Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15INK4b, a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.
AB - Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15INK4b, a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.
KW - 7SK snRNP
KW - CDK inhibitor
KW - Larp7
KW - P-TEFb
KW - Primordial germ cell
KW - Transcription
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U2 - 10.1101/gad.202242.112
DO - 10.1101/gad.202242.112
M3 - Article
C2 - 23154982
AN - SCOPUS:84869201258
VL - 26
SP - 2477
EP - 2482
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 22
ER -