Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells

Daiji Okamura; Ikuma Maeda; Hirofumi Taniguchi; Yuko Tokitake; Makiko Ikeda; Keiko Ozato; Nathan Mise; Kuniya Abe; Toshiaki Noce; Juan Carlos Izpisua Belmonte; Yasuhisa Matsui

doi:10.1101/gad.202242.112

Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells

Daiji Okamura, Ikuma Maeda, Hirofumi Taniguchi, Yuko Tokitake, Makiko Ikeda, Keiko Ozato, Nathan Mise, Kuniya Abe, Toshiaki Noce, Juan Carlos Izpisua Belmonte, Yasuhisa Matsui

Cell Resource Center for Biomedical Research

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15^INK4b, a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.

Original language	English
Pages (from-to)	2477-2482
Number of pages	6
Journal	Genes and Development
Volume	26
Issue number	22
DOIs	https://doi.org/10.1101/gad.202242.112
Publication status	Published - 2012 Nov 15

Keywords

7SK snRNP
CDK inhibitor
Larp7
P-TEFb
Primordial germ cell
Transcription

ASJC Scopus subject areas

Genetics
Developmental Biology

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Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells. / Okamura, Daiji; Maeda, Ikuma; Taniguchi, Hirofumi; Tokitake, Yuko; Ikeda, Makiko; Ozato, Keiko; Mise, Nathan; Abe, Kuniya; Noce, Toshiaki; Belmonte, Juan Carlos Izpisua; Matsui, Yasuhisa.

In: Genes and Development, Vol. 26, No. 22, 15.11.2012, p. 2477-2482.

Research output: Contribution to journal › Article › peer-review

Okamura, Daiji ; Maeda, Ikuma ; Taniguchi, Hirofumi ; Tokitake, Yuko ; Ikeda, Makiko ; Ozato, Keiko ; Mise, Nathan ; Abe, Kuniya ; Noce, Toshiaki ; Belmonte, Juan Carlos Izpisua ; Matsui, Yasuhisa. / Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells. In: Genes and Development. 2012 ; Vol. 26, No. 22. pp. 2477-2482.

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AU - Ikeda, Makiko

AU - Ozato, Keiko

AU - Mise, Nathan

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AU - Noce, Toshiaki

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AU - Matsui, Yasuhisa

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AB - Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15INK4b, a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.

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Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells

Abstract

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ASJC Scopus subject areas

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