Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells

Daiji Okamura; Ikuma Maeda; Hirofumi Taniguchi; Yuko Tokitake; Makiko Ikeda; Keiko Ozato; Nathan Mise; Kuniya Abe; Toshiaki Noce; Juan Carlos Izpisua Belmonte; Yasuhisa Matsui

doi:10.1101/gad.202242.112

Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells

Daiji Okamura, Ikuma Maeda, Hirofumi Taniguchi, Yuko Tokitake, Makiko Ikeda, Keiko Ozato, Nathan Mise, Kuniya Abe, Toshiaki Noce, Juan Carlos Izpisua Belmonte, Yasuhisa Matsui

Cell Resource Center for Biomedical Research

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15^INK4b, a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.

Original language	English
Pages (from-to)	2477-2482
Number of pages	6
Journal	Genes and Development
Volume	26
Issue number	22
DOIs	https://doi.org/10.1101/gad.202242.112
Publication status	Published - 2012 Nov 15

Keywords

7SK snRNP
CDK inhibitor
Larp7
P-TEFb
Primordial germ cell
Transcription

ASJC Scopus subject areas

Genetics
Developmental Biology

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title = "Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells",

abstract = "Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15INK4b, a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.",

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author = "Daiji Okamura and Ikuma Maeda and Hirofumi Taniguchi and Yuko Tokitake and Makiko Ikeda and Keiko Ozato and Nathan Mise and Kuniya Abe and Toshiaki Noce and Belmonte, {Juan Carlos Izpisua} and Yasuhisa Matsui",

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AU - Okamura, Daiji

AU - Maeda, Ikuma

AU - Taniguchi, Hirofumi

AU - Tokitake, Yuko

AU - Ikeda, Makiko

AU - Ozato, Keiko

AU - Mise, Nathan

AU - Abe, Kuniya

AU - Noce, Toshiaki

AU - Belmonte, Juan Carlos Izpisua

AU - Matsui, Yasuhisa

PY - 2012/11/15

Y1 - 2012/11/15

N2 - Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15INK4b, a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.

AB - Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15INK4b, a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.

KW - 7SK snRNP

KW - CDK inhibitor

KW - Larp7

KW - P-TEFb

KW - Primordial germ cell

KW - Transcription

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Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells

Abstract

Keywords

ASJC Scopus subject areas

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