Cell-based electrochemical assay for endotoxin using a secreted alkaline phosphatase reporter system

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13 Citations (Scopus)

Abstract

We developed a cell-based assay device for the detection of endotoxin, the potentially toxic compound that induces septic shock. Genetically-engineered cells that secrete alkaline phosphatase (SEAP) on exposure to endotoxin were cultured in an electrochemical cell device in medium containing p-aminophenyl phosphate and various concentrations of endotoxin. After 24 hr incubation, p-aminophenol (pAP), generated by SEAP-catalyzed hydrolysis, was detected by amperometry at + 0.35 V. The amperometric response increased with the concentration of endotoxin in the range of 0.01-1 ng/ml.

Original languageEnglish
Pages (from-to)525-528
Number of pages4
JournalElectrochemistry
Volume76
Issue number8
DOIs
Publication statusPublished - 2008 Aug

Keywords

  • Amperometry
  • Cell-based sensor
  • Lipopolysaccharide
  • Secreted alkaline phosphatase

ASJC Scopus subject areas

  • Electrochemistry

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