Cell-associated collagenolytic activity by Candida albicans

Masahiro Nishimura, Hiroki Nikawa, Hirofumi Yamashiro, Haruki Nishimura, Taizo Hamada, Graham Embery

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Cell associated collagenolytic activity of Candida albicans was quantified by measuring the degradation of synthetic peptide 2-furanacryloyl-Leu-Gly-Pro-Ala (FALGPA), which is a specific substrate for collagenase, by the freeze-thaw procedure method. This collagenolytic activity was enhanced by cells cultured in the presence of bovine serum albumin (BSA) in culture medium. However, this activity was inhibited in the presence of ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), but not by the serine proteinase inhibitor p-amidinophenyl methanesulfonyl fluoride (APMSF), nor the aspartyl proteinase inhibitor pepstatin A. These results suggested the presence of a metalloenzyme on pericellular C. albicans.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
Issue number3
Publication statusPublished - 2002
Externally publishedYes


  • Candida albicans
  • Collagenolytic activity
  • Root caries

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science
  • veterinary (miscalleneous)


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