Cell adhesion control on photoreactive phospholipid polymer surfaces

Batzaya Byambaa, Tomohiro Konno, Kazuhiko Ishihara

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Non-invasive and effective cell recovery from culture substrates is important for the passage and characterization of cells. In this study, a photoreactive polymer surface, which uses UV-irradiation to control substrate cell adhesion, was prepared. The photoreactive phospholipid polymer (PMB-PL) reported herein, was composed of a both 2-methacryloyloxyethyl phosphorylcholine (MPC) unit as a cytocompatible unit and methacrylate bearing a photolabile nitrobenzyl group. The PMB-PL polymer was used to coat a cell culture substrate thus affording a photoreactive surface. Surface analysis of the PMB-PL coating indicated a strong photoresponse owing to the sensitivity of the PL unit. Before light exposure, the PMB-PL surface provided cell adhesion. Following UV-irradiation, the PMB-PL coating was converted to a neutral ζ-potential and hydrophilic surface. The photoreactive surface conversion process allowed for the detachment of adhered cells from the PMB-PL surface while maintaining cell viability. This study demonstrates the promise and significance of the PMB-PL photoreactive surface as a method to control cell attachment and detachment for cell function investigation.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume99
DOIs
Publication statusPublished - 2012 Nov 1
Externally publishedYes

Keywords

  • Cell attachment
  • Cell detachment
  • Phospholipid polymer
  • Photocleavable
  • Photoreactive polymer
  • Stimuli-responsive

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Fingerprint

Dive into the research topics of 'Cell adhesion control on photoreactive phospholipid polymer surfaces'. Together they form a unique fingerprint.

Cite this