cDNA cloning of mitochondrial Δ3, Δ2-enoyl-CoA isomerase of rat liver

Yoshihisa Tomioka; Akihiko Hirose; Hiroshi Moritani; Takanori Hishinuma; Takashi Hashimoto; Michinao Mizugaki

doi:10.1016/0167-4781(92)90473-D

cDNA cloning of mitochondrial Δ³, Δ²-enoyl-CoA isomerase of rat liver

Yoshihisa Tomioka, Akihiko Hirose, Hiroshi Moritani, Takanori Hishinuma, Takashi Hashimoto, Michinao Mizugaki

PHA - Pharmacy

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A 1.08 kbp cDNA encoding rat liver mitochondrial Δ³, Δ²-enoyl-CoA isomerase (ECI) of 298 amino acid residues (M_r 32 895) was isolated from rat liver λgt11, λgt10 cDNA libraries by the combination of an immunochemical method with a rabbit-antibody against rat liver ECI and a plaque hybridization method. The deduced amino acid sequence from the cDNA indicates that ECI is synthesized with an amino-terminal extrasequence of 35 amino acid residues and processed to the mature enzyme (M_r 29 256).

Original language	English
Pages (from-to)	109-112
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	1130
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(92)90473-D
Publication status	Published - 1992 Feb 28

Keywords

(Rat liver)
cDNA
Δ, Δ-Enoyl-CoA isomerase
β-Oxidation
λgt10
λgt11

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

Access to Document

10.1016/0167-4781(92)90473-D

Cite this

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title = "cDNA cloning of mitochondrial Δ3, Δ2-enoyl-CoA isomerase of rat liver",

abstract = "A 1.08 kbp cDNA encoding rat liver mitochondrial Δ3, Δ2-enoyl-CoA isomerase (ECI) of 298 amino acid residues (Mr 32 895) was isolated from rat liver λgt11, λgt10 cDNA libraries by the combination of an immunochemical method with a rabbit-antibody against rat liver ECI and a plaque hybridization method. The deduced amino acid sequence from the cDNA indicates that ECI is synthesized with an amino-terminal extrasequence of 35 amino acid residues and processed to the mature enzyme (Mr 29 256).",

keywords = "(Rat liver), cDNA, Δ, Δ-Enoyl-CoA isomerase, β-Oxidation, λgt10, λgt11",

author = "Yoshihisa Tomioka and Akihiko Hirose and Hiroshi Moritani and Takanori Hishinuma and Takashi Hashimoto and Michinao Mizugaki",

year = "1992",

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T1 - cDNA cloning of mitochondrial Δ3, Δ2-enoyl-CoA isomerase of rat liver

AU - Tomioka, Yoshihisa

AU - Hirose, Akihiko

AU - Moritani, Hiroshi

AU - Hishinuma, Takanori

AU - Hashimoto, Takashi

AU - Mizugaki, Michinao

PY - 1992/2/28

Y1 - 1992/2/28

N2 - A 1.08 kbp cDNA encoding rat liver mitochondrial Δ3, Δ2-enoyl-CoA isomerase (ECI) of 298 amino acid residues (Mr 32 895) was isolated from rat liver λgt11, λgt10 cDNA libraries by the combination of an immunochemical method with a rabbit-antibody against rat liver ECI and a plaque hybridization method. The deduced amino acid sequence from the cDNA indicates that ECI is synthesized with an amino-terminal extrasequence of 35 amino acid residues and processed to the mature enzyme (Mr 29 256).

AB - A 1.08 kbp cDNA encoding rat liver mitochondrial Δ3, Δ2-enoyl-CoA isomerase (ECI) of 298 amino acid residues (Mr 32 895) was isolated from rat liver λgt11, λgt10 cDNA libraries by the combination of an immunochemical method with a rabbit-antibody against rat liver ECI and a plaque hybridization method. The deduced amino acid sequence from the cDNA indicates that ECI is synthesized with an amino-terminal extrasequence of 35 amino acid residues and processed to the mature enzyme (Mr 29 256).

KW - (Rat liver)

KW - cDNA

KW - Δ, Δ-Enoyl-CoA isomerase

KW - β-Oxidation

KW - λgt10

KW - λgt11

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