Abstract
A 1.08 kbp cDNA encoding rat liver mitochondrial Δ3, Δ2-enoyl-CoA isomerase (ECI) of 298 amino acid residues (Mr 32 895) was isolated from rat liver λgt11, λgt10 cDNA libraries by the combination of an immunochemical method with a rabbit-antibody against rat liver ECI and a plaque hybridization method. The deduced amino acid sequence from the cDNA indicates that ECI is synthesized with an amino-terminal extrasequence of 35 amino acid residues and processed to the mature enzyme (Mr 29 256).
| Original language | English |
|---|---|
| Pages (from-to) | 109-112 |
| Number of pages | 4 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 1130 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1992 Feb 28 |
Keywords
- (Rat liver)
- cDNA
- Δ, Δ-Enoyl-CoA isomerase
- β-Oxidation
- λgt10
- λgt11
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics
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Tomioka, Y., Hirose, A., Moritani, H., Hishinuma, T., Hashimoto, T., & Mizugaki, M. (1992). cDNA cloning of mitochondrial Δ3, Δ2-enoyl-CoA isomerase of rat liver. BBA - Gene Structure and Expression, 1130(1), 109-112. https://doi.org/10.1016/0167-4781(92)90473-D