Abstract
A 1.08 kbp cDNA encoding rat liver mitochondrial Δ3, Δ2-enoyl-CoA isomerase (ECI) of 298 amino acid residues (Mr 32 895) was isolated from rat liver λgt11, λgt10 cDNA libraries by the combination of an immunochemical method with a rabbit-antibody against rat liver ECI and a plaque hybridization method. The deduced amino acid sequence from the cDNA indicates that ECI is synthesized with an amino-terminal extrasequence of 35 amino acid residues and processed to the mature enzyme (Mr 29 256).
Original language | English |
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Pages (from-to) | 109-112 |
Number of pages | 4 |
Journal | BBA - Gene Structure and Expression |
Volume | 1130 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1992 Feb 28 |
Keywords
- (Rat liver)
- cDNA
- Δ, Δ-Enoyl-CoA isomerase
- β-Oxidation
- λgt10
- λgt11
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics