cDNA cloning of a new low-density lipoprotein receptor-related protein and mapping of its gene (LRP3) to chromosome bands 19q12-q13.2

Hirofumi Ishii, Dong Ho Kim, Teizo Fujita, Yuichi Endo, Shigeru Saeki, Tokuo T. Yamamoto

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

We report herein the cDNA cloning of a novel member of the low-density lipoprotein receptor (LDLR) family termed LDLR-related protein 3 (LRP3). Human and rat LRP3 cDNAs encode a 770-amino-acid type 1 membrane protein with the following regions: a putative signal sequence, two isoleucine/leucine/valine-rich regions with an RGD sequence, two ligand- binding repeat regions, a putative transmembrane region, and a proline-rich cytoplasmic region with a tyrosine-based internalization signal. Fluorescence in situ hybridization of human chromosomes revealed that the human LRP3 gene mapped to chromosome bands 19q12-q13.2. LRP3 transcript was detected in a wide range of human tissues, with the highest expression in skeletal muscle and ovary. Despite the presence of the ligand-binding repeats, CHO cells transfected with LRP3 did not bind β-migrating very-low-density lipoprotein or receptor-associated protein.

Original languageEnglish
Pages (from-to)132-135
Number of pages4
JournalGenomics
Volume51
Issue number1
DOIs
Publication statusPublished - 1998 Jul 1

ASJC Scopus subject areas

  • Genetics

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