cDNA cloning and structure of mouse putative Ah receptor

M. Ema, K. Sogawa, N. Watanabe, Y. Chujoh, N. Matsushita, O. Gotoh, Y. Funae, Y. Fujii-Kuriyama

    Research output: Contribution to journalArticlepeer-review

    331 Citations (Scopus)

    Abstract

    Mouse cDNA clones for a putative Ah receptor have been isolated from a cDNA library of mRNA from Hepa-1 cells by an oligonucleotide probe produced by PCR with a pair of primers which was synthesized according to the reported N-terminal sequence of 26 amino acids. The cDNA clones encode a polypeptide of 805 amino acids with a helix-loop-helix motif and with some similarity to a certain region designated PAS of Drosophila Per and Sim, and human Arnt protein. Cotransfection of an expression vector of the Ah receptor with a reporter plasmid pMC6.3k consisting of CYP1A1 promoter and CAT structural gene into CV-1 cells enhanced the CAT expression in response to added 3-methylcholanthrene.

    Original languageEnglish
    Pages (from-to)246-253
    Number of pages8
    JournalBiochemical and biophysical research communications
    Volume184
    Issue number1
    DOIs
    Publication statusPublished - 1992 Apr 15

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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